ROLE OF PALASH FLOWER (Butea monosperma) POWDER IN IMPROVING THE COLOUR STABILITY OF ORNAMENTAL KOI CARP (Cyprinus carpio L, 1758)
DOI:
https://doi.org/10.48165/abr.2025.27.01.26Keywords:
Cost-effective feed, Koi carp, palash flower, pigmentationAbstract
The colour of ornamental fish significantly influences their market value, with Koi carp (Cyprinus carpio) being globally admired for their vibrant patterns. Palash flower (Butea monosperma), commonly known as the flame of forest, is a natural carotenoid source abundantly available during April–May in Midnapore, Bankura, and Purulia districts of West Bengal, India. During flowering, large quantities of flowers fall to the ground and often go to waste. This study evaluated the effect of dietary incorporation of palash flower powder on the skin and muscle pigmentation of Koi carp. The 60-day indoor aquarium experiment involved six groups: T1 (control, no supplementation), T2 (2.5%), T3 (5%), T4 (7.5%), T5 (10%), and T6 (commercial feed). Fish were fed twice daily at 4% of body weight, with experimental feeds prepared using Pearson’s square method. Pigment concentration was determined spectrophotometrically. The highest total carotenoid content in skin (25.09 ± 0.20 µg g⁻¹) and muscle (15.78 ± 1.6 µg g⁻¹) was observed in T4 (p < 0.05), which also recorded the highest specific growth rate, weight gain, and 100% survival. Results indicate that 7.5% palash flower supplementation offers optimal pigmentation, growth performance, and cost-effectiveness compared to control and commercial diets.
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Alma, A., del Villar-Martinez, J., Orbe-Rogel, E. C., Vanegas-Espinoza, P., Quintero-Gutierrez, A. G., & Lara Flores, M. (2013). The effect of marigold (Tagetes erecta) as natural carotenoid source for the pigmentation of goldfish (Carassius auratus L.). Research Journal of Fisheries and Hydrobiology, 8(2), 31–37.
Andriani, Y., Wulandari, A. P., Pratama, R. I., & Zidni, I. (2016). Pengaruh penambahan tepung Spirulina pada pakan buatan terhadap kualitas warna ikan mas koi (Cyprinus carpio). Prosiding Seminar Nasional Perikanan dan Kelautan, 5(1), 133–143.
AOAC. (2007). Official methods of analysis (18th ed.). Association of the Official Analytical Chemists, Arlington, Virginia, USA.
Boonyarapalin, M., & Lovell, R. T. (1977). Diet preparation for aquarium fish. Aquaculture, 12, 53–62.
Boonyaratpalin, M., & Unprasert, N. (1989). Effect of pigments from different sources on colour changes and growth of red Oreochromis niloticus. Aquaculture, 79, 375–380.
Chapman, F. A. (2000). Ornamental fish culture, freshwater. In R. E. Stickney (Ed.), Encyclopedia of aquaculture (pp. 602–610). John Wiley & Sons.
Das, A. P., & Biswas, S. P. (2020). The effect of ripe papaya, Carica papaya, as natural carotenoids meal on body pigmentation and growth performance in banded gourami, Trichogaster fasciata. International Journal of Aquatic Biology, 8(2), 83–90.
Ezhil, J., Jeyanthi, C., & Narayanan, M. (2008). Marigold as a carotenoid source on pigmentation and growth of red swordtail, Xiphophorus helleri. Turkish Journal of Fisheries and Aquatic Sciences, 8, 99–102.
Ghiasvand, Z., & Shapouri, M. (2006). The effect of synthetic and natural pigments on the colour of Albino Oscar (Astronotus ocellatus sp., Agassiz, 1831). Journal of Marine Biology, 1, 75–83.
Gomelsky, B., Cherfas, N., Hulata, G., & Dasgupta, S. (2003). Inheritance of the white-red (Kohaku) clor complex in ornamental (Koi) carp (Cyprinus carpio L.). Israeli Journal of Aquaculture - Bamidgeh, 55(3), 147–153.
Goodwin, T. W. (1984). Carotenoid-protein complexes. In The biochemistry of the carotenoids (Vol. 2, pp. 1–21).
Gupta, S., Mehla, K., Chauhan, D., & Nair, A. (2011). Anti-inflammatory activity from leaves of Michelia champaca investigated on acute inflammation induced rats. Latin American Journal of Pharmacy, 30(4), 819–822.
Jagadeesh, T. D., Shivananda Murthy, H., Swain, H., Chetan, N., Manjunatha, A. R., & Baglodi, V. (2014). Effect of marigold oleoresin on growth, survival and pigmentation in orange chromide, Etroplus maculatus (Bloch, 1795). Fishery Technology, 51, 25–30.
Jagadeesh, T. D., Murthy, H. S., Surendranath, S. V., Panikkar, P. R., Manjappa, N., & Maesh, V. (2015). Effects of supplementation of marigold (Tagetes erecta) oleoresin on growth, survival and pigmentation of rosy barb, Puntius conchonius (Hamilton). An Internal Quarterly Journal of Life Sciences, 10(3), 1431–1435.
Jain, A., & Kaur, V. I. (2016). Rose petal meal as a potential natural carotenoid source for pigmentation and growth of freshwater ornamental fish, koi carp, Cyprinus carpio (Linnaeus). Indian Journal of Ecology, 43(1), 239–244.
Kalinowki, C. T., Robaina, L. E., Fernandez-Palacios, H., Schuchardt, D., & Izquierdo, M. S. (2005). Effect of different carotenoid sources and their dietary levels on red porgy (Pagrus pagrus) growth and skin colour. Aquaculture, 244, 223–231.
Mukherjee, R., & Maity, J. (2024). Colour retention in ornamental fish, Indian rosy barb (Pethia conchonius), using petal meal of Asian pigeonwings (Clitoria ternatea). Applied Biological Research, 26(1), 78–83.
Olson, J. A. (1979). A simple dual assay for vitamin A and carotenoids in human liver. Nutrition Reports International, 19(6), 807–813.
Ramamoorthy, K., Bhuvaneswari, S., Sankar, G., & Sakkaravarthi, K. (2010). Proximate composition and carotenoid content of natural carotenoid sources and its colour enhancement on marine ornamental fish Amphiprion ocellaris (Cuveir, 1880). World Journal of Fish and Marine Sciences, 2(6), 545–550.
Sinha, K., Saha, P. D., & Datta, S. (2012). Extraction of natural dye from petals of flame of forest (Butea monosperma) flower: Process optimization using response surface methodology (RSM). Dyes and Pigments, 94(2), 212–216.
Sitorus, A. M. G., & Nurmatias Usman, S. (2015). The effect of astaxanthin concentration on feed on the increase in the color of goldfish (Carassius auratus). Journal of Aquacoastmarine, 8(3), 10.
Wagner, J. J., & Stanton, T. L. (2012). Formulating rations with the Pearson square – 1.618. Colorado State University Extension, Colorado, USA.
