CHARCATERIZATION AND GROWTH KINETICS OF Phomopsis psidii CAUSING STYLER END ROT OF GUAVA (Psidium guava)
DOI:
https://doi.org/10.48165/abr.2025.27.01.45Keywords:
Fungal biomass, growth kinetics, guava, styler end rot, Phomopsis psidii, physiological studiesAbstract
Styler end rot is one of the most significant diseases affecting the styler end of guava fruit, causing direct yield losses. The present study aimed to characterize the causal pathogen of styler end rot, Phomopsis psidii, on a physiological basis and to assess its growth kinetics (ΔrAUKC) under laboratory conditions. The effects of different nutrient media, temperature regimes, pH levels, carbon sources, nitrogen sources, and trace elements on fungal growth were evaluated. Among six nutrient media tested, potato dextrose agar (PDA) supported the highest diametric growth (90 mm) and growth rate (0.59 mm h⁻¹), producing white mycelium with a ring pattern. Maximum diametric growth (90 mm) and higher growth rates (0.56–0.59 mm h⁻¹) were observed at 25°C and pH 6.5 after 144 hours of incubation. Peak fungal growth occurred between 48 and 72 hours of incubation, irrespective of nutrient media, temperature, or pH. In terms of biomass production, starch was the most effective carbon source (1141.87 mg), potassium nitrate was the best nitrogen source (282.30 mg), and magnesium sulphate proved to be the most effective trace element (858.33 mg). Understanding the optimal growth conditions of Phomopsis psidii provides a foundation for developing targeted cultural and chemical management strategies against guava styler end rot.
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Benson, D.A., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. and Sayers, E.W. (2013). GenBank. Nucleic Acids Research, 42 (Database issue), D32. https://doi.org/10.1093/nar/gks1195
Bhogal, S., Jarial, K., Jarial, R.S. and Banyal, S.K. (2022). Cultural analysis and growth kinetics of Pestaliopsis psidii (Pat.) Mordue causing scabby fruit canker in guava (Psidium guajava L.). Indian Phytopathology, 28(2), 53–160.
Bills, G.F., Platas, G., Fillola, A., Jimenez, M.R., Collado, J., Vicente, F., et al. (2008). Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays. Journal of Applied Microbiology, 104(6), 1644–1658.
Bita, C.E. and Gerats, T. (2013). Plant tolerance to high temperature in a changing environment: Scientific fundamentals and production of heat stress-tolerant crops. Frontiers in Plant Science, 4, 273. https://doi.org/10.3389/fpls.2013.00273
Borhade, A.K. (2023). Eco-friendly management of styler end rot (Phomopsis psidii) disease of guava (Psidium guajava L.). MSc Thesis, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, India.
Costa, E., Teixido, N., Usall, J., Atares, E. and Vinas, I. (2002). The effect of nitrogen and carbon sources on growth of the biocontrol agent Pantoea agglomerans strain CPA‐2. Letters in Applied Microbiology, 35(2), 117–120.
Drais, M.I., Rossini, L., Turco, S., Faluschi, A. and Mazzaglia, A. (2023). Modelling germination and mycelium growth rates of Monostichella coryli under constant temperature conditions. Fungal Ecology, 61, 101201. https://doi.org/10.1016/j.funeco.2022.101201
Falandysz, J. (2021). Nutritional and other trace elements and their associations in raw King Bolete mushrooms (Boletus edulis). International Journal of Environmental Research and Public Health, 19(1), 417. https://doi.org/10.3390/ijerph19010417
Fang, Q.H. and Zhong, J.J. (2002). Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderma lucidum. Biotechnology Progress, 18(1), 51–54.
Girish, K. and Bhat, S. (2011). Physiological variability among isolates of Phomopsis azadirachtae from Tamil Nadu. Journal of Yeast and Fungal Research, 2(5), 65–74.
Granjo, C.A., dos Reis, T.A., Gambale, W. and Correa, B. (2007). Morphogenesis and growth kinetics of Fusarium verticillioides. Mycopathologia, 164, 119–126.
Gurgel, L.M.S., Menezes, M. and Coelho, R.S.B. (2002). A comparative study of Phomopsis anacardii and Phomopsis mangiferae isolates by pathogenicity and nutrition of carbon and nitrogen under three light systems. Summa Phytopathologica, 28, 160–166.
Gwon, J.H., Park, H. and Eom, A.H. (2022). Effect of temperature, pH, and media on the mycelial growth of Tuber koreanum. Mycobiology, 50(4), 238–243.
Jakatimath, S.P., Mesta, R.K., Mushrif, S.K., Biradar, I.B. and Ajjappalavar, P.S. (2017). In vitro evaluation of fungicides, botanicals and bioagents against Phomopsis vexans, the causal agent of fruit rot of brinjal. Journal of Pure and Applied Microbiology, 11(1), 229–235.
Karaoglanidis, G.S. and Bardas, G. (2006). First report of Phomopsis fruit decay on apple caused by Phomopsis mali in Greece. Plant Disease, 90(3), 375. https://doi.org/10.1094/PD-90-0375C
Kaur, B. and Atri, N.S. (2016). Effect of growth regulators and trace elements on the vegetative growth of Pleurotus sapidus Qual. International Journal of Pharmacy and Pharmaceutical Sciences, 8(11), 283–287.
Kupradit, C., Ranok, A., Mangkalanan, S., Khongla, C. and Musika, S. (2020). Effects of natural carbon sources and temperature on mycelium cultivations of edible mushrooms. Thai Journal of Science and Technology, 9(4), 539–553.
Lazarevic, J., Stojicic, D. and Keca, N. (2016). Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest. Forest Systems, 25(1), e048.
Mahadevakumar, S. and Janardhana, G.R. (2016). Leaf blight and fruit rot disease of brinjal caused by Diaporthe vexans (Phomopsis vexans) in six agro-ecological regions of South West India. Plant Pathology and Quarantine, 6(1), 5–12.
Maqsood, A., Rehman, A., Ishtiaq, A., Muhammad, N., Jamil, M., Rahmatullah, Q., et al. (2014). Physiological attributes of fungi associated with stem end rot of mango (Mangifera indica L.) cultivars in postharvest fruit losses. Pakistan Journal of Botany, 46(5), 1915–1920.
Marin, E.A., Casas, A., Landrum, L., Shock, M.P., Sizzo, H.A., Sanchez, E.R., et al. (2021). The taming of Psidium guajava: Natural and cultural history of a neotropical fruit. Frontiers in Plant Science, 12, 714763. https://doi.org/10.3389/fpls.2021.714763
Misra, A.K. and Pandey, B.K. (2013). Guava (Psidium guajava L.). In: Singh, H.P., Anandraj, M. and Bhat, A.I. (eds.), Advances in Horticulture Biotechnology Diagnostics for Horticulture Crops, pp. 108–119. CHAI, Westville, New Delhi, India.
Nag Raj, T.R. and Ponnappa, K.M. (1974). Phomopsis psidii. In: Beiträge zur Kenntnis der Gattung Phomopsis. Beihefte zur Nova Hedwigia, 47, 547. https://www.gbif.org/species/5481538
Parvathy, P.S., Sanjeev, T.V., Swami, M., Venugopal, B. and Merson, M. (2023). The effect of different physical factors on growth and morphology of six pathogenic fungal species. International Journal of Scientific Research in Engineering and Management, 7(1), 1–33.
Patil, P.D., Baikar, A.A. and Raut, R.A. (2016). Performance of different growth media for the growth and sporulation of Phomopsis sp. of cashew. Plant Archives, 16(1), 275–278.
Pawar, A.B. (2021). Effect of nutrients on cellulolytic and pectolytic enzyme production of Phomopsis viticola, a leaf spot pathogen of grapes (Vitis vinifera). International Journal of Biological Science, 3(1), 18–20.
Rai, J.N. (1956). Styler end rot of the guava fruit (Psidium guayava Linn.). Springer India, 43(1), 55–61.
Robinson, J.R., Isikhuemhen, O.S. and Anike, F.N. (2021). Fungal-metal interactions: A review of toxicity and homeostasis. Journal of Fungi, 7(3), 225. https://doi.org/10.3390/jof7030225
Rohini, Gowtham, H.G., Hariprasad, P. and Niranjana, S.R. (2016). Cultural, morphological and physiological characterization of Phomopsis vexans isolates from different brinjal (Solanum melongena L.) growing regions of Karnataka. International Journal of Pharma and Bio Sciences, 7(3), 70–77.
Roy, P., Mandal, R., Pan, S.K. and Tarafdar, J. (2014). Impact of carbon and nitrogen sources on Phomopsis vexans isolates. Trends in Biosciences, 7(12), 1222–1226.
Rozsa, M., Maniuțiu, D.N. and Egyed, E. (2021). Influence of magnesium (Mg) source on the Cordyceps militaris (L.) mushroom mycelium growth. Current Trends in Natural Sciences, 10, 333–340.
Selvig, K. and Alspaugh, J.A. (2011). pH response pathways in fungi: Adapting to host-derived and environmental signals. Mycobiology, 39(4), 249–256.
Sharma, D., Jarial, K. and Jarial, R.S. (2025). Effect of different carbon, nitrogen sources and trace elements on mycelial biomass production of Colletotrichum gloeosporioides causing anthracnose in citrus. International Journal of Economic Plants, 12(2). https://doi.org/10.23910/2/2025.6036a
Sharma, R. and Rajak, R.C. (2003). Keratinophilic fungi: Nature’s keratin degrading machines! Their isolation, identification and ecological role. Resonance, 8, 28–40.
Sharma, S., Jarial, R.S. and Jarial, K. (2025). In vitro evaluation of different nutritive media for mycelial growth of Pleurotus eryngii (DC. ex Fr.) Quel. Indian Journal of Ecology, 52(1), 103–106.
Srivastava, S., Pathak, N. and Srivastava, P. (2011). Identification of limiting factors for the optimum growth of Fusarium oxysporum in liquid medium. Toxicology International, 18(2), 111. https://doi.org/10.4103/0971-6580.84262
Sugha, S.K., Kaushal, N. and Kumar, S. (2012). Factors affecting development of Phomopsis fruit rot of brinjal. Indian Phytopathology, 55(1), 26–29.
Sumra, N.H., Shabbir, N., Naureen, N., Muhammad, P. and Madiha, R. (2018). The phytochemistry and medicinal value of Psidium guajava. Clinical Phytoscience, 4(1), 32. https://doi.org/10.1186/s40816-018-0093-8
Thiyam, B. and Sharma, G.D. (2014). In vitro impact of temperature on the radial growth of pathogenic fungi. Indian Journal of Applied Research, 4(2), 4–5.
Tian, Y., Zhao, Y., Sun, T., Wang, L., Liu, J., Ma, X. and Hu, B. (2018). Identification and characterization of Phomopsis amygdali and Botryosphaeria dothidea associated with peach shoot blight in Yangshan, China. Plant Disease, 102(12), 2511–2518.
Valkovic, T. and Damic, M.S. (2022). Role of iron and iron overload in the pathogenesis of invasive fungal infections in patients with hematological malignancies. Journal of Clinical Medicine, 11(15), 4457. https://doi.org/10.3390/jcm11154457
Van, T.C., Nguyen, T.T. and Pham, H.T. (2005). Morphological and molecular characterization of Phomopsis species associated with fruit rot. Asian Journal of Mycology, 19(1), 45–57.
Walker, G.M. and White, N.A. (2017). Introduction to fungal physiology. In: Kavanagh, K. (ed.), Fungi: Biology and Applications, pp. 1–35. John Wiley, Chichester, UK.
Wang, B.T., Hu, S., Yu, X.Y., Jin, L., Zhu, Y.J. and Jin, F.J. (2020). Studies of cellulose and starch utilization and the regulatory mechanisms of related enzymes in fungi. Polymers, 12(3), 530. https://doi.org/10.3390/polym12030530
Wei, F., Ma, N., Haseeb, H.A., Gao, M., Liu, X. and Guo, W. (2022). Insights into structural and physicochemical properties of maize starch after Fusarium verticillioides infection. Journal of Food Composition and Analysis, 114, 104819. https://doi.org/10.1016/j.jfca.2022.104819
White, T.J., Bruns, T., Lee, S.B. and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, pp. 315–322. Academic Press, New York, USA.
Wiriya, J., Kavinlertvatana, P. and Lumyong, S. (2014). Effects of different culture media, carbon and nitrogen sources and solid substrates on growth of Termitomyces mushrooms. Chiang Mai Journal of Science, 41(3), 542–556.
Wrona, C.J., Mohankumar, V., Schoeman, M.H., Tan, Y.P., Shivas, R.G., Jeff-Ego, O.S., et al. (2020). Phomopsis husk rot of macadamia in Australia and South Africa caused by novel Diaporthe species. Plant Pathology, 69(5), 911–921.
Xie, F.L., Zhou, X.Y., Xiao, R., Zhang, C.J., Zhong, J., Zhou, Q.Z., et al. (2022). Discovery and exploration of widespread infection of mycoviruses in Phomopsis vexans, the causal agent of phomopsis blight of eggplant in China. Frontiers in Plant Science, 13, 996862. https://doi.org/10.3389/fpls.2022.996862
