Harnessing native antagonistic bio-agents for eco-friendly control of rice sheath rot caused by Sarocladium oryzae
DOI:
https://doi.org/10.48165/jefa.2025.20.2.27Keywords:
Rice sheath rot, Trichoderma, Pseudomonas, antagonistic bio-agents, sustainable agricultureAbstract
Rice sheath rot, caused by Sarocladium oryzae, is a devastating disease threatening rice production worldwide, with yield losses ranging from 9.6 per cent to 85 per cent. Conventional reliance on synthetic fungicides has raised concerns about environmental sustainability and health safety, prompting the exploration of eco-friendly alternatives. This study investigates the in vitro efficacy of native antagonistic bio-agents, specifically Trichoderma spp. and Pseudomonas spp., against Sarocladium oryzae. Rhizospheric soil samples were collected to isolate and identify bio-agents, which were then tested using the dual culture method. Results revealed significant pathogen inhibition, with Trichoderma isolate Tr-1 achieving the highest inhibition rate (76.72%) among the Trichoderma isolates and in Pseudomonas isolates Ps-1 (61.21%) showed the significant inhibition. These findings highlight the potential of native bio-agents as sustainable, environmentally friendly alternatives to chemical fungicides, offering a promising strategy for integrated disease management in rice cultivation and advancing organic farming practices.
Downloads
References
Agnihothrudu, V. (1973). Acrocylindrium oryzae Sawada—Sheath rot on paddy. Kavaka, 1, 69–71.
Bag, M. K., Roychoudhury, U. K., & Adhikari, B. (2010). Evaluation of botanicals and biopesticides against sheath rot disease of rice. Journal of Crop and Weed, 6, 78–79.
Barnett, H. L., & Binder, F. L. (1973). The fungal host-parasite relationship. Annual Review of Phytopathology, 11, 273–292.
Dennis, C., & Webster, J. (1971). Antagonistic properties of species groups of Trichoderma. I. Production of non-volatile antibiotics. Transactions of the British Mycological Society, 57, 25–41.
Eziashi, E. I., Uma, N. U., Adekunle, A. A., & Airede, C. E. (2006). Effect of metabolites produced by Trichoderma species against Ceratocystis paradoxa in culture medium. African Journal of Biotechnology, 5, 703–706.
Gams, W., & Hawksworth, D. L. (1975). The identity of Acrocylindrium oryzae Sawada and a similar fungus causing sheath-rot of rice. Kavaka, 3, 57–61.
Gopalakrishnan, C., & Valluvaparidasan, V. (2006). Seed-borne biocontrol agents for the management of rice sheath rot caused by Sarocladium oryzae (Sawada) W. Gams & D. Hawksw. Journal of Biological Control, 20, 197–204.
Harman, G. E., Lo, C. T., Nelson, E. B., & Hayes, C. K. (1998). Ecological studies of transformed Trichoderma harzianum strain 1295–22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology, 88, 129–136.
Kalaiselvi, S. S., & Panneerselvam, A. (2011). In vitro assessment of antagonistic activity of Trichoderma sp. against Sarocladium oryzae causing sheath rot disease in paddy. International Journal of Applied Biology and Pharmaceutical Technology, 4, [ISSN: 0976–4550].
Martínez, B., Obret, Y., Pérez, S., & Reyes, Y. (2014). Antagonism in vitro of Trichoderma spp. against Sarocladium oryzae (Sawada) W. Gams & D. Hawksworth. Revista de Protección Vegetal, 29, 106–111.
Mew, T. W., & Misra, J. K. (Eds.). (1994). A manual of rice seed health testing. International Rice Research Institute.
Panneerselvam, A., & Saravanamuthu, R. (1996). Antagonistic interaction of some soil fungi against Sarocladium oryzae. Indian Journal of Agricultural Research, 30, 59–64.
Sanchez, V., Rebolledo, O., Picaso, R. M., Cardenas, E., Cordova, J., & Samuels, G. J. (2006). In vitro antagonism of Theieloviopsis paradoxa by Trichoderma longibrachiatum. Mycopathologia, 163, 49–58.
Shekhada, M. R., Patil, V. A., Savaliya, A. S., & Sangani, M. D. (2018). Management of rice (Oryza sativa L.) sheath blight (Rhizoctonia solani Kuhn) and sheath rot (Sarocladium oryzae Sawada) through seed bio-priming. International Journal of Current Microbiology and Applied Sciences, 7, 2787–2794.
Srinivas, P., & Ramakrishnan, G. (2003). Effect of volatile and non-volatile metabolites produced by native microorganisms in biocontrol of rice seedborne pathogens. Annals of Plant Protection Sciences, 11, 53–56.
Tasugi, H., & Ikeda, Y. (1956). Studies on the sheath rot of rice plants caused by Acrocylindrium oryzae Sawada. Bulletin of the National Institute of Agricultural Sciences, Tokyo, 6, 151–166.
Vincent, J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159(4051), 850–850.
