Enhancing the Storability of Coated Broccoli Heads Using Irradiated Chitosan Combined with Olive Leaves Extract

Authors

  • Amr M Mounir Natural Products Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority Author
  • A M El-Hefny Natural Products Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority. Author
  • Asmaa Ezz El-Dein Food Irradiation Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority Author
  • Israa F M Eldehn Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority. Author

DOI:

https://doi.org/10.48165/jntas.2025.13.1.5

Keywords:

Broccoli, Irradiated Chitosan, Olive Leaves Extract.

Abstract

Broccoli is one of the most important nontraditional vegetable crops in Egypt. It gains its importance from its high nutritional value, but there are some obstacles facing broccoli storage such as florets yellowing, high respiration rate which lead to increase weight loss and chlorophyll degradation. thus, this experiment was carried out during the two successive seasons of 2023/2024 and 2024/2025 to evaluate the effect of both un-irradiated and irradiated chitosan (CS) with 25kGy gamma rays combined with 1 and 2% of olives leaves extract (OLE) as an edible coating to enhance the storage ability of broccoli heads under cold storage conditions (5o and 90-95% RH). Data revealed that, broccoli heads coated with irradiated chitosan with 25kGy combined with 1% followed by 2% of OLE retained the highest quality and freshness for up to 28 days, recording the the lowest weight loss percentage and decay score compared to un-coated broccoli heads. Also, it was observed that coated heads scored higher general appearance, pigment concentration (chlorophyll a, b and total chlorophyll), total soluble phenols and total free amino acids concentrations as well as total antioxidant percentage compared to uncoated broccoli heads. Thus, OLE incorporated into irradiated CS coating; relatively preserves the nutritional quality of broccoli heads during storage period. 

 

References

Abdalla, C.U., Mussagy, G., Sant’Ana Pegorin Brasil, M., Scontri, J.C., da Silva Sasaki, Y., Su, C., Bebber, R.R., Rocha, A.P., de Sousa Abreu, R.P., Goncalves, B.S., Burd, M.F., Pacheco, K.M., Romeira, F.P., Picheli, N.B., Guerra, N., Farhadi, J.F., Floriano, S., Forster, S., He, H.T., Nguyen, A., Peirsman, Z., Tirp´akov´a, S., Huang, M.R., Dokmeci, E.S., Ferreira, L.S., dos Santos, R.D., Piazza, R.F.C., Marques, A., Gom´ez, V., Jucaud, B., Li, H.M.C., & Herculano, R.D. (2023). Eco-sustainable coatings based on chitosan, pectin, and lemon essential oil nano emulsion and their effect on strawberry preservation. Int. J. Biol. Macromol., 249, 126016.

Aubry, S., Mani, J., & Hörtensteiner, S. (2008). Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide an oxygenase in the chlorophyll catabolic pathway. Plant Mol. Biol., 67(3), 243.

Chen, W., Li, J., Sun, W., Qiu, L., Yu, D., Li, N., & Ji, X. (2024). Schiff base and coordinate bonds cross-linked chitosan-based eutectogels with ultrafast self-healing, self-adhesive, and anti-freezing capabilities for motion detection. Int. J. Biol. Macromol., 257, 128434.

Clodoveo, M.L., Crupi, P., Annunziato, A., & Corbo, F. (2022). Innovative extraction technologies for development of functional ingredients based on polyphenols from olive leaves. Foods, 11(1), 103.

Development Team, M. S. T. A. T. (1989). MSTAT user’s guide: A microcomputer program for the design management and analysis of agronomic research experiments. East Lansing, USA: Michigan State University.

Duncan, D.B. (1955). Multiple range and multiple F test. Journal of Biometrics, 11, 1–42.

El Sayed, N., Hasanin, M.S., & Abdelraof, M. (2022). Utilization of olive leaves extract coating incorporated with zinc/selenium oxide nanocomposite to improve the postharvest quality of green beans pods. Bioact. Carbohydr. Diet. Fibre., 28, 100333.

EL-Bauome, H.A., Abdeldaym, E.A., Abd El-Hady, M.A.M., Darwish, D.B.E., Alsubeie, M.S., El-Mogy, M.M., Basahi, M.A., Al-Qahtani, S.M., Al-Harbi, N.A., & Alzuaibr, F.M. (2022). Exogenous proline, methionine, and melatonin stimulate growth, quality, and drought tolerance in cauliflower plants. Agriculture, 12(9), 301.

FAO Statistics. (2021). Production year book. Food and Agriculture Organization.

Garcia, M.A., de la Paz, N., Castro, C., Rodriguez, J.L., Rapado, M., Zuluaga, R., Ganan, P.F., & Casariego Ano, A. (2015). Effect of molecular weight reduction by gamma irradiation on the antioxidant capacity of chitosan from lobster shells. J. Rad. Res. Appl. Sci., 8(2), 190.

Gulluce, M., Sokmen, M., Sahin, F., Sokmen, A., Adiguzel, A., & Ozer, H. (2004). Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J. Sci. Food Agric., 84(7), 735.

Guo, L., Zhu, Y., & Wang, F. (2018). Calcium sulfate treatment enhances bioactive compounds and antioxidant capacity in broccoli sprouts during growth and storage. Postharvest Biol. Technol., 139, 12.

Hansen, M.E., Sørensen, H., & Cantwell, M. (2001). Changes in acetaldehyde, ethanol and amino acid concentrations in broccoli florets during air and controlled atmosphere storage. Postharvest Biol. Technol., 22(3), 227.

Harley, M.M., & Fergusen, I.K. (1990). The role of SEM in pollen morphology and plant systematic. Association Special, 41, 45.

Hernández, P.F.A., Yuste, M.C.A., González-Gómez, D., Gil, D.B., Delgado-Adámez, J., & García, M.J.B. (2017). Behavior of fresh cut broccoli under different modified atmosphere conditions. Emir. J. Food Agric., 29(3), 188.

Herrero, M., Temirzoda, T.N., Segura-Carretero, A., Quirantes, R., Plaza, M., & Ibañez, E. (2011). New possibilities for the valorization of olive oil by-products. J. Chromatog. A, 1218(42), 7511.

Hussein, N.M., Abdallah, M.M.F., Abou El-Yazied, A., & EL-Bassiouny, R.E.I. (2020). Effect of some edible coating and packaging on quality attributes of broccoli florets during cold storage. Arab Univ. J. Agric. Sci., 82(2), 547.

Jayaraman, J. (1985). Postharvest biological control. Wiley Eastern Limited, New Delhi.

Jimenez, M., Trejo, E., & Cantwell, M. (1998). Postharvest quality changes in green beans. Research Report, UC Davis, Cooperative Extension Service, 9.

Jongsri, P., Wangsomboondee, T., Rojsitthisak, P., & Seraypheap, K. (2016). Effect of molecular weights of chitosan coating on postharvest quality and physicochemical characteristics of mango fruit. LWT-Food Sci. Techn., 73(1), 28.

Kabir, A.H., Ela, E.J., Bagchi, R., Rahman, M.A., Peiter, E., & Lee, K.W. (2023). Nitric oxide acts as an inducer of Strategy-I responses to increase Fe availability and mobilization in Fe-starved broccoli (Brassica oleracea var. oleracea). Plant Physiol. Biochem., 194(5), 182.

Kaewsuksaeng, S., Yamauchi, N., Funamoto, Y., Shigyo, M., & Kanlavanarat, S. (2006). Effect of Mg-dechelation activity on chlorophyll degradation in stored broccoli florets. In Proceedings of the IV International Conference on Managing Quality in Chains-The Integrated View on Fruits and Vegetables Quality in Chains MQUIC, Bangkok, Thailand, International Society for Horticultural Science: Leuven, Belgium, 712, 705.

Khalatbary, A.R., & Zarrinjoei, G.R. (2012). Anti-inflammatory effect of oleuropein in experimental rat spinal cord trauma. IRCMJ, 14(4), 229.

Khalifa, I., Barakat, H., El-Mansy, H.A., & Soliman, S.A. (2016). Enhancing the keeping quality of fresh strawberry using chitosan-incorporated olive processing wastes. Food Biosc., 13(1), 69.

Kheiri, A., Jorf, M.S.A., Malihipour, A., Saremi, H., & Nikkhah, M. (2016). Application of chitosan and chitosan nanoparticles for the control of Fusarium head blight of wheat (Fusarium graminearum) in vitro and greenhouse. Int. J. Biol. Macromol., 93(Part A), 1261.

Khwaldia, K., Attour, N., Matthes, J., Beck, L., & Schmid, M. (2022). Olive byproducts and their bioactive compounds as a valuable source for food packaging applications. Compr. Rev. Food Sci. Food Saf., 21(2), 1218.

Mahmoud, T.Sh.M., Yassin, N.M.A., & Shaaban, F.K.M. (2017). Influence of postharvest application with chitosan and some natural plant extracts on storage life and quality attributes of navel orange fruits during cold storage. Middle East J. Agric. Res., 6(2), 330.

Manzanares, P., Ruiz, E., Ballesteros, M., Negro, M.J., Gallego, F.J., López-Linares, J.C., & Castro, E. (2017). Residual biomass potential in olive tree cultivation and olive oil industry in Spain: valorization proposal in a biorefinery context. Span. J. Agric. Res., 15(3), 1.

Molina-Alcaide, E., & Yáñez-Ruiz, D.R. (2008). Potential use of olive by-products in ruminant feeding: A review. Anim. Feed Sci. Technol., 147(1–3), 247.

Montes-Ramírez, P., Monta˜no-Leyva, Blancas-Benitez, F.J., Bautista-Rosales, P.U., Ruelas-Hern´andez, N.D., Martínez-Robinson, K., & González-Estrada, R.R. (2024). Active films and coatings based on commercial chitosan with natural extracts addition from coconut by-products: physicochemical characterization and antifungal protection on tomato fruits. Food Cont., 155, 110077.

Moran, R. (1982). Formulae for determination of chlorophyllous pigments extracted with N,N-Dimthylformamide. Plant Physiol., 69(6), 1376.

Moreria, M., Roura, S., & Ponce, A. (2011). Effectiveness of chitosan edible coatings to improve microbiological and sensory quality of fresh cut broccoli. LWT-Food Sci. Technol., 44(10), 2335.

Ocloo, F.C.K., Quayson, E.T., Adu-Gyamfi, A., Quarcoo, E.A., Asare, D.Y., Serfor-Armah, & Woode, B.K. (2011). Physicochemical and functional characteristics of radiation processed shrimp chitosan. Rad. Physiol. Chem., 80(7), 837.

Pereira, A.P., Ferreira, I.C., Marcelino, F., Valentao, P., Andrade, P.B., & Seabra, R. (2007). Phenolic compounds and antimicrobial activity of olive (Olea Europaea L. Cv. Cobran cosa) leaves. Molecules, 12(5), 1153.

Pintos, F.M., Hasperué, J.H., Ixtaina, P., Vicente, A.R., Lemoine, M.L., & Rodoni, L.M. (2021). Short light exposure preserves broccoli head quality and nutrients during refrigerated storage. J. Food Process. Preserv., 45(10), 15801.

Risse, L.A., & Miller, W.R. (1986). Individual film wrapping of Florida cucumbers, eggplant, peppers and tomatoes for extending shelf life. J. Plast Film. Sheet., 2(2), 163.

Samad, M., Sajid, M., Hussain, I., Samad, N., & Jan, N. (2019). Influence of herbal extract and storage duration on fruit quality of China lime. Horticul. Int. J., 3(3), 153.

Selim, S., Albqmi, M., Al-Sanea, M.M., Alnusaire, T.S., Almuhayawi, M.S., Abd Elgawad, H., Al Jaouni, S.K., Elkellish, A., Hussein, S., Warrad, M., & El-Saadony, M.T. (2022). Valorizing the usage of olive leaves, bioactive compounds, biological activities, and food applications: a comprehensive review. Front. Nutr., 9, 1008349.

Sharkawy, A., Barreiro, M.F., & Rodrigues, A.E. (2020). Chitosan-based Pickering emulsions and their applications: A Review. Carbohyd. Polym., 250, 116885

Swain, T., & Hillis, W.E. (1959). The phenolic constituents of Prunus domestica. I – The quantitative analysis of phenolic constituents. J. Sci. Food Agric., 10(1), 63.

Taha, A.M., & Khalifa, H.E.H. (2025). Olive in Egypt: Cultural Practices. Olives and olive related products-innovations in production and processing. Inn. Prod. Proc., 35.

Watada, A.E., & Morris, L.L. (1966). Effect of chilling and non-chilling temperatures on snap beans fruits. Proc. Am. Soc. Hortic. Sci., 89, 368.

Zahran, A.A., Hassanein, R.A., & Abdel Wahab, A.T. (2015). Effect of chitosan on biochemical composition and antioxidant activity of minimally processed ‘Wonderful’ pomegranate arils during cold storage. J. Appl. Bot. Food Qual., 88, 241.

Zhang, C., Li, W., Zhu, B., Chen, H., Chi, H., Li, L., Qin, Y., & Xue, J. (2018). The quality evaluation of post-harvest strawberries stored in Nano-Ag packages at refrigeration temperature. Polymers, 10(8), 894.

Zhang, Y.X., Ma, Y.L., Guo, Y.Y., Chen, Y., Yang, M., Fu, R.Q., & Sun, Y.P. (2022). Physiological and ITRAQ-based proteomic analyses for yellowing of postharvest broccoli heads under elevated O2 controlled atmosphere. Sci. Hortic., 294, 110769.

Downloads

Published

2025-09-18

How to Cite

Enhancing the Storability of Coated Broccoli Heads Using Irradiated Chitosan Combined with Olive Leaves Extract. (2025). Journal of Nuclear Technology in Applied Science, 13(1), 39-50. https://doi.org/10.48165/jntas.2025.13.1.5