Effect of Ashwagandha, Turmeric and Antibiotic on Blood Biochemical Profile of Repeat Breeding Gir Cows with Subclinical Endometritis

Authors

  • Dhruv V. Vadher Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh, Gujarat, India
  • Suryakant S. Parikh Cattle Breeding Farm, Kamdhenu University, Junagadh, Gujarat, India
  • Arun K. Sharma Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh, Gujarat, India
  • Hardipsinh P. Vijyeta Cattle Breeding Farm, Kamdhenu University, Junagadh, Gujarat, India
  • Meet N. Thummar Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh, Gujarat, India
  • Dilip R. Ram Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Junagadh, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.21.2.02

Keywords:

Gir cows, Biochemical profile, Subclinical endometritis, Therapeutic regimens

Abstract

The present study was conducted to evaluate the influence of ashwagandha and turmeric on blood biochemical profile in subclinical endometritis (SCE) affected repeat breeding Gir cows. Thirty apparently healthy repeat breeder Gir cows, aged 4 to 8 years (>90 days in milk) and positive for SCE on cytobrush technique, were selected from the University Farm and nearby areas of Junagadh region. They were randomly divided into 5 equal groups. In Group A (positive control), cows were administered 30 mL of normal saline intrauterine (I/U) as a single dose. Group B received 30 mL of moxifloxacin I/U for 3 consecutive days. In Group C and D, cows were treated with 5 mL of ashwagandha and 5 mL of turmeric extract diluted in 30 mL of distilled water, respectively, while Group E received a combination treatment of 5 mL each of ashwagandha and turmeric extract, diluted in 30 mL of distilled water and administered I/U for 3 consecutive days. Blood samples collected at estrus before treatment (0 hr) and at subsequent estrus after treatment were compared for changes in blood biochemical profile. Following treatment, significant increase (p<0.05) in blood glucose, calcium, phosphorus, and TAC levels, and decrease in serum urea and SAA concentrations were noted across all SCE affected groups, whereas serum lipid peroxidation (LPO) decreased non-significantly (p>0.05). The highest first service conception rate was observed in the group treated with the combination of ashwagandha and turmeric extract (66.66%), followed by turmeric extract alone (50.00 %), ashwagandha extract alone (33.33%) sensitive antibiotic (33.33%), and control (0.00%). The study revealed that a combination of ashwagandha and turmeric extract has best therapeutic efficacy and can be used as an alternative therapy for the treatment of subclinical endometritis in repeat breeding cows.

Downloads

Download data is not yet available.

References

Ahmadi, M.R., Mogheiseh, A., & Nazifi, S. (2018). Changes in biomarkers serum amyloid-A and haptoglobin following treatment of endometritis in dairy cows. Comparative Clinical Pathology, 27(6), 1659-1665.

Bannikov, G.A., Hinds, C.A., Rajala-Schultz, P.J., Premanandan, C., Rings, D.M., & Lakritz, J. (2011). Serum haptoglobin–matrix metalloproteinase 9 (Hp-MMP 9) complex as a biomarker of systemic inflammation in cattle. Veterinary Immunology and Immunopathology, 139(1), 41-49.

Barlund, C.S., Carruthers, T.D., Waldner, C.L., & Palmer, C. W. (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69(6), 714-723.

Biswal, S.S., Das, S., Balasubramanian, S., Mohanty, D.N., Sethy, K., & Dasgupta, M. (2014). Serum amyloid- A and haptoglobin levels in crossbred cows with endometritis following different therapy. Veterinary World, 7(12), 1066-1070.

Bradford, B.J., Yuan, K., Farney, J.K., Mamedova, L.K., & Carpenter, A. J. (2015). Invited review: Inflammation during the transition to lactation: New adventures with an old flame. Journal of Dairy Science, 98(10), 6631-6650.

Brodzki, P., Kostro, K., Krakowski, L., & Marczuk, J. (2015). Inflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum period. Veterinary Research Communications, 39, 143-149.

Chapwanya, A., Meade, K.G., Doherty, M.L., Callanan, J.J., & O’Farrelly, C. (2013). Endometrial epithelial cells are potent producers of tracheal antimicrobial peptide and serum amyloid-A3 gene expression in response to E. coli stimulation. Veterinary Immunology and Immunopathology, 151(1-2), 157-162.

Chaudhari. R.J. (2018). Diagnostic and therapeutic methods in postpartum endometritis and its impact on reproductive performance in crossbred cows. Ph.D. Thesis. Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, India.

Chaudhari, R.J., Gulavane, S.U., Rangnekar, M.N., Shelar, R.R., Gaikwad, S.M., & Jawale, R.S. (2020). Subclinical endometritis in crossbred cows with special reference to blood biochemistry and reproductive parameters, Haryana Veterinarian, 59(SI), 41-44.

Das, S., Mishra, S.K., Swain, R.K., Mohanty, D.N., & Mishra, S.R. (2012). Comparative study of certain serum biochemical parameters in anoestrus and repeat breeding cows of Bhadrak district of Orissa. The Indian Journal of Field Veterinarians, 7(4), 62-65.

Gopikrishnan, D., Selvaraju, M., Palanisamy, M., Periyannan, M., Ravikumar, K., & Senthilkumar, K. (2022). Therapeutic efficacy of garlic (Allium sativum), turmeric (Curcuma longa) and eucalyptus (Eucalyptus globules) extracts in the treatment of bovine endometritis. Indian Journal of Animal Research, 56(7), 904-908.

Heidarpour, M., Mohri, M., Fallah-Rad, A.H., Shahreza, F.D., & Mohammadi, M. (2012). Oxidative stress and trace elements before and after treatment in dairy cows with clinical and subclinical endometritis. Revue de Medicine Veterinaire, 163(12), 628-633.

Janowski, T., Baranski, W., Lukasik, K., Skarzynski, D., Rudowska, M., & Zdunczyk, S. (2013). Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumour necrosis factor-α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis. Polish Journal of Veterinary Sciences, 16(4), 693-699.

Kaya, S., Ogun, M., Ozen, H., Kuru, M., Sahin, L., Kukurt, A., & Kacar, C. (2017). The impact of endometritis on specific oxidative stress parameters in cows. Journal of the Hellenic Veterinary Medical Society, 68(2), 231-236.

Kumar, A., Gupta, H.P., & Prasad, S. (2018). Studies on the immunomodulatory and therapeutic efficacy of turmeric (Curcuma longa) on endometritis in repeat breeding crossbred cows. International Journal of Agriculture Sciences, 10(2), 5069-

5072.

Lawange, S.R., Markandeya, N.M., Kumawat, B.L., Sawale, A.G., Anbhule, R.S., & Kadam, P.D. (2019). Efficacy of garlic extract±ashwagandha for the treatment of infectious repeat breeding in cattle. The Indian Journal of Animal Reproduction, 40(1), 31-34.

Mahour, S.S., Nema, S.P., Aich, R., Karmore, S.K., Jatav, G.P., & Kurechiya, N. (2021). Use of ethanolic herbal extracts and ciprofloxacin for the treatment of subclinical endometritis

in crossbred cows. International Journal of Livestock Research, 11(1), 177-183.

Pandey, V., Singh, A.K., & Sharma, N. (2009). Blood biochemical profile in fertile and repeat breeding crossbred cows under field conditions. Veterinary Practitioner, 10(1), 45-48.

Parikh, S.S. (2021). Comparative studies on different diagnostic methods and therapeutic approaches for postpartum subclinical endometritis in Gir cows. Ph. D. Thesis. Kamdhenu University, Junagadh, Gujarat, India.

Pascottini, O.B., Hostens, M., Sys, P., Vercauteren, P., & Opsomer, G. (2017). Cytological endometritis at artificial insemination in dairy cows: Prevalence and effect on pregnancy outcome. Journal of Dairy Science, 100(1), 588-597.

Perumal, P., Chaurasia, D., De, A.K., Bhattacharya, D., Sunder, J., Bhowmick, S., Kundu, A., & Mishra, P.C. (2020). Effect of clinical endometritis on physiological, haematological, biochemical and endocrinological profiles in crossbred cows under tropical island ecosystem. Indian Journal of Animal Sciences, 90(9), 1296-1299.

Pothmann, H., Prunner, I., Wagener, K., Jaureguiberry, M., De la Sota, R.L., Erber, R., Aurich, C., Ehling-Schulz, M., & Drillich, M. (2015). The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. Theriogenology, 83(8), 1249-1253.

Raje, A., Kumar, N., Ranjan, R., Singh, A.K., Singh, S.K., Agrawal, A., & Singh, A.P. (2023). Evaluation of haematological parameters

treated with moxifloxacin on uterine infection in bovines. International Journal of Veterinary Sciences and Animal Husbandry, 8(4), 293-295.

Salah, N., Khudhair, N.Y., Rasheed, Y., & Mahmood, M.Y. (2021). Comparison of serum acute phase proteins and inflammatory cytokines between healthy and subclinically endometritic postpartum buffaloes. Journal of Animal & Plant Sciences, 31(3), 671-680.

Singh, B., Gupta, H.P., Shiv Prasad, S., & Singh, G.K. (2018). Effect of uterine defence modulation on recovery and conception rate in endometritic repeat breeding crossbred cows. International Journal of Current Microbiology and Applied Sciences, 7, 105-116.

Singh, N., Singh, B., & Kumar, R. (2023). Modulation of serum cholesterol, AST, glucose and fertility outcomes following uterine lavage, and parenteral administration of levamisole, cloprostenol and their combinations in endometritic buffaloes. Ruminant Science, 12(1), 153-156.

Verma, R.K., Kumari, P., Maurya, R.K., Kumar, V., Verma, R.B., & Singh, R.K. (2018). Medicinal properties of turmeric (Curcuma longa L.): A review. International Journal of Chemical Studies, 6(4), 1354-1357.

Wu, H., Dai, A., Chen, X., Yang, X., Li, X., Huang, C., Jiang, K., & Deng, G. (2018). Leonurine ameliorates the inflammatory responses in lipopolysaccharide-induced endometritis. International Immunopharmacology, 61, 156-161.

Downloads

Published

2025-02-26

Issue

Vol. 21 No. 2 (2025): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2025

Section

Research Article

License

Copyright (c) 2025 Indian Journal of Veterinary Sciences and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

V. Vadher, D., S. Parikh , S., K. Sharma, A. ., P. Vijyeta, H. ., N. Thummar, . M. ., & R. Ram, D. (2025). Effect of Ashwagandha, Turmeric and Antibiotic on Blood Biochemical Profile of Repeat Breeding Gir Cows with Subclinical Endometritis . Indian Journal of Veterinary Sciences and Biotechnology, 21(2), 7-12. https://doi.org/10.48165/ijvsbt.21.2.02
Crossref
Scopus
Google Scholar
Europe PMC