Comparative Analysis of Intradermal Tuberculin Test and Polymerase Chain Reaction for Bovine Tuberculosis Detection in Punjab, India

Authors

  • Sudhir Kumar Prajapati Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Deepti Narang Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Mudit Chandra Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Sikh Tejinder Singh Department of Animal Genetics & Breeding, College of Veterinary Science, GADVASU, Ludhiana-141004, Punjab, India
  • Ashwani Sharma Department of Veterinary Medicine, College of Veterinary Science, GADVASU, Ludhiana-141004, Punjab, India

DOI:

https://doi.org/10.48165/ijvsbt.21.2.09

Keywords:

Bovine, Comparative intradermal tuberculin test, Mycobacterium kansasii, Non-tuberculous mycobacteria, PCR.

Abstract

Bovine tuberculosis (bTB) is a zoonotic disease that affects domestic animals, humans, and the wild animals. The purpose of the study was to use the comparative intradermal tuberculin test to diagnose bTB in cattle and to find out the prevalence in unorganized, and organized farm with variation according to age, sex, and breed. Eighty three animals were tested with the use of comparative intradermal tuberculin test across two districts of Punjab, India. Additionally, ante-mortem (nasal swab, n=44) samples were examined using molecular methods. The samples were inoculated on Middlebrook7H10 media after decontamination with 4% NaOH. One sample revealed the presence of acid fast bacilli on microscopic examination. Out of 83 animals examined, 12 were bTB positive reactors. Additionally, gender-wise data analysis revealed higher positivity among cows/heifers (16.17%, 11/68) compared to bulls/bullocks (6.66%, 1/15). Out of the 44 nasal swab samples, 3 samples yielded Mycobacterium kansasii isolates on culture which were further confirmed by species specific PCR. The finding of our study indicates animals of indigenous cattle breed in Ludhiana have a higher prevalence of bTB and variation in breed susceptibility. The presence of non-tuberculous mycobacteria may hamper diagnosing bovine tuberculosis in cattle.

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References

Ameni, G., Vordermeier, M., Firdessa, R., Aseffa, A., & Hewinson, G. (2011). M. tuberculosis infection in grazing cattle in central Ethiopia. The Veterinary Journal, 188, 359-3561.

Bezos, J., Alvarez, J., Romero, B., De Juan, L., & Dominguez, L. (2014). Bovine tuberculosis: Historical perspective. Research in Veterinary Science, 97, 3-4.

Boland, F., Kelly, G.E., Good, M., & More, S.J. (2010). Bovine tuberculosis and milk production in infected dairy herds in Ireland. Preventive Veterinary Medicine, 93(2-3), 153-161.

Brooks-Pollock, E., Conlan, A. J., Mitchell, A. P., Blackwell, R., McKinley, T. J., & Wood, J. L. (2013). Age-dependent patterns of bovine tuberculosis in cattle. Veterinary Research, 44, 1-9.

Chang, C.L., Kim, H.H., Son, H.C., Park, S.S., Lee, M.K., Park, S.K., & Jeon, C.H. (2001). False-positive growth of Mycobacterium tuberculosis attributable to laboratory contamination confirmed by restriction fragment length polymorphism

Fig. 4: Agarose gel electrophoresis showing an amplicon of 439 bp (hsp65 gene) from nasal swabs samples: Lane M: 100 bp DNA ladder, Lane L1 and L3-L4: Positive for hsp65 gene.

analysis. The International Journal of Tuberculosis and Lung Disease, 5(9), 861-867.

Coad, M., Clifford, D.J., Vordermeier, H.M., & Whelan, A.O. (2013). The consequences of vaccination with the Johne’s disease vaccine, Gudair, on diagnosis of bovine tuberculosis. Veterinary Record, 172(10), 266-266.

Daley, C.L., Iaccarino, J.M., & Lange, C. (2020). Treatment of nontuberculous mycobacterial pulmonary disease: An Official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline (vol 71, pg e1, 2020). Clinical Infectious Diseases, 71(11), 3023-3023.

Das, R., Dandapat, P., Chakrabarty, A., Nanda, P. K., Bandyopadhyay, S., & Bandyopadhyay, S. (2018). A cross-sectional study on prevalence of bovine tuberculosis in Indian and crossbred cattle in Gangetic delta region of West Bengal, India. International Journal of One Health, 4(7), 2018-2021.

Dhinakaran, M., Appaji Rao, V.N., & Nedunchellian, S. (1991). Immunological response in tuberculin reactor cattle. In: Compendium of Decennial Convention of ISVM and National Symposium on recent advances in control of diseases of crossbred and companion animals, 19-21 December, Bombay Veterinary College, Goregaon, Mumbai, India.

Dinka, H., & Duressa, A. (2011). Prevalence of bovine tuberculosis in Arsi Zones of Oromia, Ethiopia. African Journal of Agricultural Research, 6(16), 3853-3858.

Dubey, S., Singh, R.V., Gupta, B., Nayak, A., Rai, A., & Gupta, D. (2020). Bovine tuberculosis and its public health significance/bovine tuberculosis and its public health significance: A review. Journal of Entomology and Zoology Studies, 8, 2281-2287.

Gcebe, N., & Hlokwe, T.M. (2017). Non-tuberculous mycobacteria in South African wildlife: Neglected pathogens and potential impediments for bovine tuberculosis diagnosis. Frontiers in Cellular and Infectious Microbiology, 7, 15.

Honda, J.R., Virdi, R., & Chan, E.D. (2018). Global environmental nontuberculous mycobacteria and their contemporaneous man-made and natural niches. Frontiers in Microbiology, 9, 2029.

Javed, M. T., Ahmad, L., Irfan, M., Ali, I., Khan, A., Wasiq, M., & Cagiola, M. (2010). Haematological and serum protein values in tuberculin reactor and non-reactor water buffaloes, cattle, sheep and goats. Pakistan Veterinary Journal, 30, 100-104.

Kankya, C., Muwonge, A., Djonne, B., Munyeme, M., & Opuda-Asibo, J. (2011). Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public health significance. BMC Public Health, 11, 1-9.

LoBue, P.A., Enarson, D.A., & Thoen, C.O. (2010). Tuberculosis in humans and animals: An overview. Tuberculosis: A re-emerging disease in animals and humans. The International Journal of Tuberculosis and Lung Disease, 14(9), 1075-1078.

Moiane, I., Machado, A., Santos, N., Nhambir, A., Inlamea, O., Hattendorf, J., & Correia-Neves, M. (2014). Prevalence of bovine tuberculosis and risk factor assessment in cattle in rural livestock areas of Govuro District in the Southeast of Mozambique. PloS One, 9(3), e91527.

Mukherjee, F. (2006). Comparative prevalence of tuberculosis in two dairy herds in India. Revue Scientifique Et Technique-Office International Des Epizooties, 25(3), 1125-1130.

Nour-Neamatollahie, A., Ebrahimzadeh, N., Siadat, S.D., Vaziri, F., Eslami, M., Sepahi, A.A., & Fateh, A. (2017). Distribution of non

tuberculosis mycobacteria strains from suspected tuberculosis patients by heat shock protein 65 PCR–RFLP. Saudi Journal of Biological Sciences, 24(6), 1380-1386.

OIE (2009). Bovine Tuberculosis. Terestial manual. World Organization for Animal Health, Paris, France.

Park, H., Jang, H., Kim, C., Chung, B., Chang, C.L., Park, S.K., & Song, S. (2000). Detection and identification of mycobacteria by amplification of the internal transcribed spacer regions with genus-and species-specific PCR primers. Journal of Clinical Microbiology, 38(11), 4080-4085.

Schiller, I., Oesch, B., Vordermeier, H.M., Palmer, M.V., Harris, B.N., Orloski, K.A., & Waters, W.R. (2010). Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transboundary and Emerging Diseases, 57(4), 205-220.

Senanayake, N.P., Eriyagama, N.B., & Thevanesam, V. (2016). Identification of non-tuberculous mycobacteria isolated from patients at Teaching Hospitals, Kandy and Peradeniya. Sri Lankan Journal of Infectious Diseases, 6(1), 33-44.

Shinnick, T.M. (1987). The 65-kilodalton antigen of Mycobacterium tuberculosis. Journal of Bacteriology, 169(3), 1080-1088. Thakur, A., Sharma, M., Katoch, V.C., Dhar, P., & Katoch, R.C. (2010). A study on the prevalence of bovine tuberculosis in farmed dairy cattle in Himachal Pradesh. Veterinary World, 3(9), 408. Vordermeier, M., Ameni, G., Berg, S., Bishop, R., Robertson, B.D., Aseffa, A., & Young, D.B. (2012). The influence of cattle breed on susceptibility to bovine tuberculosis in Ethiopia. Comparative Immunology, Microbiology and Infectious Diseases, 35(3), 227- 232.

Yu, X.L., Lu, L., Chen, G.Z., Liu, Z.G., Lei, H., & Song, Y.Z. (2014). Identification and characterization of non-tuberculous mycobacteria isolated from tuberculosis suspects in Southern central China. PloS One, 9(12), 114353.

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Published

2025-02-26

Issue

Vol. 21 No. 2 (2025): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2025

Section

Research Article

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Copyright (c) 2025 Indian Journal of Veterinary Sciences and Biotechnology

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How to Cite

Kumar Prajapati, S., Narang, D., Chandra, M., Tejinder Singh, S., & Sharma, A. (2025). Comparative Analysis of Intradermal Tuberculin Test and Polymerase Chain Reaction for Bovine Tuberculosis Detection in Punjab, India . Indian Journal of Veterinary Sciences and Biotechnology, 21(2), 43-47. https://doi.org/10.48165/ijvsbt.21.2.09
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