Intracytoplasmic Motile Sperm Selection in Fresh and Frozen Semen and its Correlation with Seminal Attributes of Kankrej Bull

Authors

  • Karan F. Chaudhary Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India
  • Babulal N. Suthar Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India
  • Vishal S. Suthar Department of Animal Biotechnology, Gujarat Biotechnology University, Gandhinagar-382355, Gujarat, India
  • Haresh C. Nakhashi Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India
  • Chaitanya G. Joshi Gujarat Biotechnology Research Centre, Gandhinagar-382010, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.21.2.16

Keywords:

Cryopreservation, IMSI, Kankrej bull,, Semen, SMPT

Abstract

Total 8 mature healthy Kankrej bulls (7 replicates) were selected for evaluation of seminal attributes at fresh and frozen stage of cryopreservation. Seminal attributes evaluated included sperm viability, acrosome integrity, HOST-EN test, intracytoplasmic motile sperm selection (IMSI) and sperm mucus penetration test (SMPT). Based on the nuclear abnormalities, sperms were classified in Grade I, II, III and IV. Per cent sperm viability, acrosome integrity, HOST- reacted sperm and Grade I sperm were found significantly lower in frozen semen as compared to fresh semen. Grade IV sperm as assessed by IMSI tool was found significantly higher in frozen semen as compared to fresh semen. Correlation study revealed significant and positive correlations of sperm viability with acrosome integrity and HOST, while significant negative correlation with grade IV sperm. Sperm viability and acrosome integrity were positively correlated with SMPT. Key words: Cryopreservation, IMSI, Kankrej bull, Semen, SMPT

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References

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Published

2025-02-26

Issue

Vol. 21 No. 2 (2025): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2025

Section

Research Article

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Copyright (c) 2025 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

F. Chaudhary, K., N. Suthar, B., S. Suthar, V., C. Nakhashi, H., & G. Joshi, C. (2025). Intracytoplasmic Motile Sperm Selection in Fresh and Frozen Semen and its Correlation with Seminal Attributes of Kankrej Bull . Indian Journal of Veterinary Sciences and Biotechnology, 21(2), 80-84. https://doi.org/10.48165/ijvsbt.21.2.16
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