Molecular Detection of Multidrug Resistant Staphylococcus aureus from Retail Meat Samples of Tirupati Town, Andhra Pradesh
DOI:
https://doi.org/10.48165/ijvsbt.21.4.05Keywords:
Antibiotic sensitivity, Food poisoning, Meat samples, , nuc gene, S. aureus.Abstract
Staphylococcus aureus causes variety of diseases in both animals and humans. Foods of animal origin may be an important source for the transfer of antimicrobial-resistant S. aureus and antimicrobial resistance genes to humans. Hence the present study was designed to detect multidrug resistant S. aureus among retail meat samples of Tirupati town, Andhra Pradesh (India). A total of 250 samples in the form of meat/swabs (chicken 100, mutton 60, beef 30, pork 30, sea meat 30) were collected from different retail outlets of Tirupati town, and processed for isolation and identification of S. aureus by cultural method and molecular characterisation by PCR. S. aureus was confirmed targeting nuc gene and all the isolates were subjected to antibiotic sensitivity test by disc diffusion method against 10 antibiotics. Results showed that out of 250 meat samples, 200 were positive for S. aureus with an overall prevalence of 80.0% (200/250) by cultural method and 68.0% (136/200) of them were confirmed by PCR. Antimicrobial sensitivity test revealed higher resistance to cefoxitin (85.29%) and ceftriaxone (66.91%) compared to other selected antibiotics. They showed high sensitivity to co-trimoxazole (66.91%) but exhibited lower sensitivity to vancomycin (8.82%). This study revealed a high prevalence of S. aureus from chicken and sea meat samples by cultural and PCR methods, respectively, which indicates that contamination of S. aureus was common in retail meat shops. By enhancing hygienic practices and quality control measures at meat processing facilities the contamination with food borne pathogens can be reduced.
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