Isolation and Molecular Detection of Fowl Adenovirus causing Inclusion Body Hepatitis utilizing Specific Pathogen- Free Embryonated Chicken Eggs

Authors

  • Mehak Tikoo Department of Veterinary Microbiology, Nagpur Veterinary College, MAFSU, Nagpur-440006, Maharashtra, India
  • Shubhangi Warke Professor & Head, Department of Veterinary Microbiology, Nagpur Veterinary College, MAFSU, Seminary Hills, Nagpur-440006, India
  • Prashant Sonkusale Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur-440006, Maharashtra, India
  • Rohan Fulsundar Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur-440006, Maharashtra, India

DOI:

https://doi.org/10.48165/ijvsbt.21.4.10

Keywords:

Chorioallantoic membrane (CAM), Chicken embryonated eggs, Hexon gene, Histopathology, nclusion body hepatitis

Abstract

Indian poultry industry is rapidly growing with an increase in demand among consumers, thus, prevailing control and prevention  measures of poultry diseases need to be improved along with the implementation of efficient disease diagnostics. Fowl adenovirus  (FAdV) is responsible for causing inclusion body hepatitis (IBH) in broiler chickens, incurring huge economic losses in the poultry  industry. A total of 11 pooled liver homogenate samples from an outbreak of IBH in a poultry farm of Katol near Nagpur were collected  and processed by conventional PCR based on the detection of hexon gene and isolation in SPF embryonated chicken eggs. Out of  11 samples, 05 (45.45%) were found to be positive for FAdV by PCR. Inoculated embryos were haemorrhagic with prominent lesions  in the liver. All the viral isolates showing characteristic pathological lesions were detected positive by PCR. Histopathology revealed  basophilic intranuclear inclusion bodies in the liver of infected embryos. Continuous surveillance of FAdVs in broiler chicken flocks is  necessary for early detection of diseases.  

 

Downloads

Download data is not yet available.

References

Abghour, S., Zro, K., Mouahid, M., Tahiri, F., Tarta, M., Berrada, J., & Kichou, F. (2019). Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco. PLOS One, 14(12), 1–14.

Alzuheir, I. M., Jalboush, N. H., Fayyad, A. F., & Daibes, R. A. (2021). Phylogenetic analysis and pathological characterization of fowl adenovirus isolated during inclusion body hepatitis outbreak in Tubas, Palestine. Veterinary Research Forum: An International Quarterly Journal, 12(4), 511–514.

Chavan, V. G., Awandkar, S. P., Kulkarni, R. C., Chavhan, S. G., Suryawanshi, R. D., Jadhav, R. K., Kulkarni, M. B., & Agnihotri, A. A. (2024). Isolation and molecular characterization of fowl adenovirus from inclusion body hepatitis cases in Western India during 2019–2021. Iranian Journal of Veterinary Research, 25(1), 62–68.

Dutta, B., Pathak, D. C., Barman, N. N., Hazarika, N., & Goswami, S. (2023). Temporo-spatial sero-epidemiology of fowl adenovirus (FAdV) infection causing inclusion body hepatitis hydropericardium syndrome (IBH-HPS) in broiler population of Assam. Indian Journal of Animal Research, 57(1), 102–107.

Islam, M. N., Rahman, M. M., Rahman, M. K., & Alam, J. (2023). First evidence of fowl adenovirus induced inclusion body hepatitis in chicken in Bangladesh. Canadian Journal of Infectious Diseases and Medical Microbiology, 2023(1), 7253433.

Kumar, A., Ranjan, K., Prasad, M., Mahajan, N. K., & Maan, S. (2022). Molecular detection, phylogenetic analysis and differentiation of fowl adenovirus from chickens with inclusion body hepatitis in Haryana. Indian Journal of Veterinary Science and Biotechnology, 18(2), 113–117.

Lebdah, M., Alshaya, D. S., Jalal, A. S., Mousa, M. R., Radwan, M. M., Samir, M., Adel, A., Albaqami, N. M., El-Saadony, M. T., El-Tarabily, K. A., & El Basrey, Y. F. H. (2022). Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens. Poultry Science, 101(12), 101918.

Meulemans, G., Boschmans, M., Berg, T. P., & Decaesstecker, M. (2001). Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathology, 30(6), 655–660.

Niczyporuk, J. S., & Kozdruń, W. (2022). Current epidemiological situation in the context of inclusion body hepatitis in poultry flocks in Poland. Virus Research, 318, 198825.

Nuñez, L., Santander-Parra, S., Catroxo, M., Astolfi-Ferreira, C. S., Loor-Giler, A., & Ferreira, A. P. (2024). Fowl adenovirus 8a isolated from chickens with runting and stunting syndrome induces inclusion body hepatitis and hepatitis-hydropericardium syndrome in chicken embryos. Veterinary World, 17(11), 2556–2566.

Rashid, F., Xie, Z., Wei, Y., Xie, Z., Xie, L., Li, M., & Luo, S. (2024). Biological features of fowl adenovirus serotype-4. Frontiers in Cellular and Infection Microbiology, 140, 1370414.

Sadekuzzaman, M., Miah, M. S., Parvin, R., Haque, M. E., Islam, T. R., Sigma, S. H., Hossain, M. G., Hayat, S., Hossain, M. T., & Islam, M. A. (2024). Pathological investigation, molecular characterization and first-time isolation of the predominant serotypes of fowl adenovirus (FAdV-D and E) from commercial poultry in Bangladesh. Frontiers in Microbiology, 15, 1490255.

Safwat, M. M., Sayed, A. S. R., Ali Elsayed, M. F., & Ibrahim, A. A. E. H. (2022). Genotyping and pathogenicity of fowl adenovirus isolated from broiler chickens in Egypt. BMC Veterinary Research, 18(1), 325.

Schachner, A., Matos, M., Grafl, B., & Hess, M. (2018). Fowl adenovirus induced diseases and strategies for their control – A review on the current global situation. Avian Pathology, 47(2), 111–126.

Shankar, K. S., Priyanka, E., Mathivanan, B., Kumar, B. P., Mukhopadhayay, S. K., Mondal, S., & Kannaki, T. R. (2022). Molecular epidemiology of fowl adenovirus (FAdV) from inclusion body hepatitis (IBH) incidences from Indian broilers revealed the prevalence of serotypes of FAdV-D and E. Indian Journal of Animal Research, 56(1), 1–6.

Shinde, D. B., Anil, L. T., Santosh, S. K., Neeti, S., Ashok, R., Vinay, R., Prasad, W., Teguh, Y. P., & Satish, S. T. (2020). Molecular and pathotypic characterization of fowl adenovirus associated with inclusion body hepatitis in Indian chickens. The Indian Journal of Animal Sciences, 90, 982–986.

Sohaimi, N. M., Bejo, M. H., Omar, A. R., Ideris, A., & Isa, N. M. (2018). Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens. Journal of Veterinary Science, 19(6), 759–770.

Suvarna, K. S., Layton, C., & Bancroft, J. D. (2018). Bancroft’s Theory and Practice of Histological Techniques (7th ed.). Elsevier Health Sciences.

Thabet, A. M., Alzuheir, I. M., Fayyad, A. F., Kheimar, A. M., & Jalboush, N. H. (2023). Occurrence and phylogenetic analysis of fowl adenovirus E in broiler flocks from Gaza Strip Palestine. The Journal of Infection in Developing Countries, 17(4), 565–570.

Wang, T., Meng, F., Chen, C., Shen, Y., Li, P., Xu, J., Feng, Z., Qu, X., Wang, F., Li, B., & Liu, M. (2023). Pathogenicity and epidemiological survey of fowl adenovirus in Shandong Province from 2021 to 2022. Frontiers in Microbiology, 14, 1166078.

Downloads

Published

2025-07-05

Issue

Vol. 21 No. 4 (2025): The Indian Journal of Veterinary Sciences and Biotechnology(IJVSBT) (JULY-AUGUEST )2025

Section

Research Article

License

Copyright (c) 2025 Indian Journal of Veterinary Sciences and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Tikoo, M., Warke, S., Sonkusale, P., & Fulsundar, R. (2025). Isolation and Molecular Detection of Fowl Adenovirus causing Inclusion Body Hepatitis utilizing Specific Pathogen- Free Embryonated Chicken Eggs. Indian Journal of Veterinary Sciences and Biotechnology, 21(4), 49-54. https://doi.org/10.48165/ijvsbt.21.4.10