Effect of Cerium Oxide Nanoparticles as an Additive in Cryopreservation of Gir Bull Spermatozoa
DOI:
https://doi.org/10.48165/ijvsbt.21.4.25Keywords:
Cerium oxide nanoparticles, Gir bull, Malondialdehyde, Post-thaw sperm quality, Total antioxidantAbstract
The present investigation was carried out on semen of four Gir bulls at Cattle Breeding Farm, Junagadh for a period of 6 months (Feb July 2024). Semen (n=16 ejaculates) was collected using artificial vagina (Danish model) once weekly from each bull. The semen was evaluated for sperm quality attributes along with oxidative stress parameters at post-thaw stage of cryopreservation using different concentrations of Cerium Oxide Nanoparticles, viz., 25 µg/mL, 50 µg/mL, 75 µg/mL CeO2NPs and a control AndroMed® extender. AndroMed® extender with 75 µg/mL CeO2NPs concentration had significantly (p<0.001) higher sperm motility, viability, HOST reactive sperm, acrosome integrity with lower sperm abnormality as compared to that of the 25 µg/mL and 50 µg/mL CeO2NPs and control extender. Mean malondialdehyde (MDA) levels in seminal plasma of post-thaw semen varied significantly between treated and control groups, but the value with 75 µg/mL CeO2NPs concentration was the lowest and it increased with the decreased level of CeO2NPs, and was highest in control group, while total antioxidants activity (TAC) increased proportionately with the increase in CeO2NPs concentration, suggesting its beneficial role as an antioxidant molecule. All the above post-thaw seminal attributes were better in extender containing CeO2NPs at 75 µg/mL concentration. The findings suggested the cryoprotective and antioxidant effects of CeO2NPs on bull spermatozoa by lowering free radicals and its associated oxidative stress.
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Al-Janabi, G. A., Al-Dulaimi, M. K., & Fadhel, A. A. (2024). Effect of adding different levels of cerium oxide nanoparticles to tris extender on some parameters of cooled semen from Holstein bulls. In IOP Conference Series: Earth and Environmental Science, 1371(7), 072046.
Bilodeau, J. F., Chatterjee, S., Sirard, M. A., & Gagnon, C. (2000). Levels of antioxidant defences are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development: Incorporating Gamete Research, 55(3), 282-288.
Bucak, M. N., Atessahin, A., & Yuce, A. (2008). Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze thawing process. Small Ruminant Research, 75(2-3), 128-134.
Bucak, M. N., Baspinar, N., Tuncer, P. B., Coyan, K., Sariozkan, S., Akalin, P. P., Buyukleblebici, S., & Kucukgunay, S. (2012). Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andrologia, 44(s-1), 102-109.
Farhadi, F., Towhidi, A., Shakeri, M., & Seifi-Jamadi, A. (2022). Zinc oxide nanoparticles have beneficial effect on frozen-thawed spermatozoa of Holstein bulls. Iranian Journal of Applied Animal Science, 12(1), 49-55.
Gaur, S., & Sharma, B. (2003). Schiff base macrocyclic ligand derived from thiosemicarbazone with their spectroscopic and antimicrobial studies. Journal of the Indian Chemical Society, 8, 841.
Hosseinmardi, M., Siadat, F., Sharafi, M., Roodbari, N. H., & Hezavehei, M. (2022). Protective effect of cerium oxide nanoparticles on human sperm function during cryopreservation. Biopreservation and Biobanking, 20(1), 24-30.
Jahanbin, R., Yazdanshenas, P., Rahimi, M., Hajarizadeh, A., Tvrda, E., Nazari, S. A., Sangcheshmeh, A. M., & Ghanem, N. (2021). In vivo and in vitro evaluation of bull semen processed with zinc nanoparticles. Biological Trace Element Research, 199(1), 126-135.
Kasimanickam, R., Kasimanickam, V., Thatcher, C. D., Nebel, R. L., & Cassell, B. G. (2007). Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology, 65(5), 1004-1012.
Khalil, W. A., El-Harairy, M. A., Zeidan, A. E., & Hassan, M. A. (2019). Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation. Theriogenology, 126, 121-127.
Khalil, W. A., Hassan, M. A., El-Harairy, M. A., & Abdelnour, S. A. (2023). Supplementation of thymoquinone nanoparticles to semen extender boosts cryotolerance and fertilizing ability of buffalo bull spermatozoa. Animals, 13(18), 2973.
Khalique, M. A., Andrabi, S. M. H., Majeed, K. A., Yousaf, M. S., Ahmad, N., Tahir, S. K., Fayyaz, Haider, M. H., Naz, S. S., Qureshi, I. Z., Sulaiman, S., Zane, H., & Rehman, H. (2024). Cerium oxide nanoparticles improve the post-thaw quality and in-vivo fertility of Beetal buck spermatozoa. Theriogenology, 214, 166-172.
Pirmohamed, T., Dowding, J. M., Singh, S., Wasserman, B., Heckert, E., Karakoti, A. S., King, J. E. S., Seal, S., & Self, W. T. (2010). Nanoceria exhibit redox state-dependent catalase mimetic activity. Chemical Communications, 46(16), 2736-2738.
Ram, D. R., Parikh, S. S., Panodara, R. J., Chaudhary, J. K., Vala, K. B., Solanki, J. Z., & Vadher, D. V. (2025). Zinc oxide nanoparticles (ZnONPs) supplementation in semen extender improves post thaw quality and antioxidant capacity of Gir bull spermatozoa. The Indian Journal of Veterinary Science and Biotechnology, 21(3), 13-17.
Snedecor, G. W., & Cochran, W. G. (1994). Statistical methods (8th ed.). Iowa State University Press.
Stadtman, E. R., & Levine, R. L. (2000). Protein oxidation. Annals of the New York Academy of Sciences, 899(1), 191-208.
Walkey, C., Das, S., Seal, S., Erlichman, J., Heckman, K., Ghibelli, L., Traversa, C., Ginnis, J. F. Mc., & Self, W. T. (2015). Catalytic properties and biomedical applications of cerium oxide nanoparticles. Environmental Science: Nano Journal, 2(1), 33-53.
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60, 481-492.
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