Withania somnifera : A Remarkable Herb to Improve Animal Fertility
DOI:
https://doi.org/10.48165/ijar.2025.46.02.3Keywords:
Ashwagandha, Aphrodisiac, Fertility, Withania somnifera, SpermAbstract
Since ancient times, herbal remedies have been utilized to treat male infertility, and in recent years, their usage has been gradually increasing. Research on the effectiveness of herbal remedies appears to be a resurgence of conventional medical methods. For more than three centuries, ashwagandha has been utilized in Ayurvedic and traditional medical systems. A rising number of human experiments are assessing ashwagandha efficacy under various situations, as a result of its growing popularity and interest in numerous Western countries. The majority of the results from these investiga tions suggested that consuming ashwagandha has benefits, despite large variations in treatment dosage, duration, and types of extract used. Overall, the reduction of stress and anxiety symptoms is the best proof of ashwagandha thera peutic effectiveness. The systematic review’s findings indicate that ashwagandha may have a wide range of medicinal uses. Ashwagandha is a widespread herb in Ayurveda that is said to enhance youth, longevity, and general well-being. Additionally, it is a potent aphrodisiac herb that supports and enhances healthy sexual function. This review will address the function of herbal remedies in male infertility and offer a thorough analysis of the numerous Withania somnifera related human and animal research that have been conducted explaining a potential direct oxidative mechanism that would reduce oxidative stress and an indirect mechanism that would enhance male fertility by modifying hormone balance through the interaction of many endocrine glands through the gamma-aminobutyric acid-like mimic pathway.
References
Ahmad, M. K., Mahdi, A. A., Shukla, K. K., Islam, N., Rajender, S., Madhukar, D., Shankhwar, S.N. and Ahmad, S. (2010). Withania somnifera improves semen quality by regulating reproductive hormone levels and oxidative stress in seminal plasma of infertile males. Fertil. Steril., 94(3): 989-996.
Alam, N., Hossain, M., Khalil, M. I., Moniruzzaman, M., Sulaiman, S. A., and Gan, S. H. (2011). High Catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis. BMC Complement. Med. Ther., 11: 1-8.
Alam, N., Hossain, M., Mottalib, M. A., Sulaiman, S. A., Gan, S. H., and Khalil, M. I. (2012). Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complement. Med. Ther., 12: 1-8.
Al‐Qarawi, A. A., Abdel‐Rahman, H. A., El‐Badry, A. A., Harraz, F., Razig, N. A., and Abdel‐Magied, E. M. (2000). The effect of extracts of Cynomorium coccineum and Withania somnifera on gonadotrophins and ovarian follicles of immature Wistar rats. Phytother. Res., 14(4): 288-290.
Ambiye, V. R., Langade, D., Dongre, S., Aptikar, P., Kulkarni, M., and Dongre, A. (2013). Clinical evaluation of the spermatogenic activity of the root extract of Ashwagandha (Withania somnifera) in oligospermic males: a pilot study. Evid. Based Complement Alternat. Med., (1): 571420.
Aslam, S., Raja, N.I., Hussain, M., Iqbal, M., Ejaz, M., Ashfaq, D., Fatima, H., Shah, M.A. and Ehsan, M. (2017). Current status of Withania somnifera (L.) Dunal: an endangered medicinal plant from Himalaya. Am. J. Plant Sci., 8(05), p.1159.
Azhar, M. F., Naseer, U., Aziz, A., Zafar, S., Qadir, I., Farooq, Ahmad, I. and Anjum, K. (2020). Antioxidant and phytochemical composition of leaves stem and root extracts of Withania coagulans and Withania somnifera. Zeitschrift für Arznei- Gewürzpflanzen., 25: 27-30.
Baghel, K., Azam, Z., and Srivastava, R. (2024). Dietary restriction‐induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis. Am. J. Reprod. Immunol., 91(2): 13816.
Bhattarai, J. P., Ah Park, S., and Han, S. K. (2010). The methanolic extract of Withania somnifera acts on GABAA receptors in gonadotropin releasing hormone (GnRH) neurons in mice. Phytother. Res., 24(8): 1147-1150.
Bolleddula, J., Fitch, W., Vareed, S. K., and Nair, M. G. (2012). Identification of metabolites in Withania somnifera fruits by liquid chromatography and high‐resolution mass spectrometry. Rapid Commun Mass Spectrom., 26(11): 1277-1290.
Dhanani, T., Shah, S., Gajbhiye, N. A., and Kumar, S. (2017). Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab. J. Chem., 10: S1193-S1199.
Dongre, S., Langade, D., and Bhattacharyya, S. (2015). Efficacy and safety of Ashwagandha (Withania somnifera) root extract in improving sexual function in women: a pilot study. Biomed Res. Int., (1): 284154.
Gamage, M. A. G. N. D. M. A., Dissanayake, W. M. N., and Yi, Y. J. (2024). Potential use of ashwagandha extract in storage of liquid boar semen. J. Biomed. Transl. Res., 25(2): 79-88.
Ilayperuma, I., Ratnasooriya, W. D., and Weerasooriya, T. R. (2002). Effect of Withania somnifera root extract on the sexual behavior of male rats. Asian J. Androl., 1(4): 295-298.,
JainR., Kachhwaha, S., and Kothari, S. L. (2012). Phytochemistry, pharmacology, and biotechnology of Withania somnifera and Withania coagulans: A review. J. Med. Plant Res., 6(41): 5388-5399.
Kalra, R., and Kaushik, N. (2017). Withania somnifera (Linn.) Dunal: a review of chemical and pharmacological diversity. Phytochem. Rev., 16: 953-987.
Kaul, K. N. (1957). The origin, distribution and cultivation of Ashwagandha the so called Withania somnifera of Indian literature. In Symposium on the Utilization of Indian Medicinal Plants. Council of Scientific & Industrial Research, New Delhi, 7-8.
Kaur, J., Seshadri, S., Golla, K. H., and Sampara, P. (2022). Efficacy and safety of standardized ashwagandha (Withania somnifera) root extract on reducing stress and anxiety in domestic dogs: A randomized controlled trial. J. Vet. Behav., 51: 8-15.
Khabiya, R., Choudhary, G. P., Jnanesha, A. C., Kumar, A., and Lal, R. K. (2024). An insight into the potential varieties of Ashwagandha (Indian ginseng) for better therapeutic efficacy. Ecol. Front., 44(3): 444-450.
Khan, S., Rai, A. K., Singh, A., Singh, S., Dubey, B. K., Lal, R. K., Negi, A.S., Birse, N., Trivedi, P.K., Elliott, C.T. and Ch, R. (2023). Rapid metabolic fingerprinting with the aid of chemometric models to identify authenticity of natural medicines: Turmeric, Ocimum, and Withania somnifera study. J. Pharm. Anal., 13(9): 1041-1057.Khare, C. P. (2008). Indian Medicinal Plants–An Illustrated Dictionary. First Indian Reprint, Springer (India) Pvt. Ltd., New Delhi 717-718.
Kumar, A., Bisla, A., Singh, A. K., Honparkhe, M., and Dhindsa, S. S. (2022). Fertility Improvement Following Herbal Treatment in Sub fertile Buffalo Bulls. Indian J. Anim. Reprod., 43(2): 54-59.
Kumar, A., Husain, D., Lal, R. K., Singh, S., Singh, V., and Gupta, A. K. (2023). Genetic diversity and future prospects in Withania somnifera (L.) Dunal: an assessment based on quantitative traits in different accessions of Ashwagandha. The Nucleus, 66(2): 151-159.
Kumar, A., Kumar, R., Rahman, M. S., Iqubal, M. A., Anand, G., Niraj, P. K., and Ali, M. (2015). Phytoremedial effect of Withania somnifera against arsenic-induced testicular toxicity in Charles Foster rats. Avicenna J. Phytomed., 5(4): 355.
Kumar, A., Venugopal, S., Jnanesha, A. C., and Lal, R. K. (2023). Agricultural-based challenges, genetic enhancement, and obstacles to an industrially important medicinal plant, Ashwagandha (Withania somnifera (L.) Dunal): A review. J. Genet. Genom., 28: 100183.
Lal, R. K., Chandra, R., Sarkar, S. S., Singh, S. S., Shasany, A. K., Gupta, A. K., Dhawan, O. P., Kumar, B., Singh, V. R., Gupta, M. M., Zaim, M., and Yadav, R. Y. (2014). Registration of high yielding variety ‘CIMAP Chetak’ suitable for medium irrigated areas of Nagori Ashwagandha (Withania somnifera L. Dunal). J. Med. Arom. Plant Sci., 36(2): 59-61.
Lal, R. K., Gupta, P., Chanotiya, C. S., Mishra, A., and Kumar, A. (2023). Eminent high essential oil yielding and photosynthesis efficient genotype selection across multi-environments in the palmarosa {Cymbopogon martinii (Roxb.) Wats. J. Genet. Genom., 27: 100167.
Mahdi, A. A., Shukla, K. K., Ahmad, M. K., Rajender, S., Shankhwar, S. N., Singh, V., and Dalela, D. (2011). Withania somnifera improves semen quality in stress‐related male fertility. Evid. Based Complement Alternat. Med., 2011(1): 576962.
Mali, P. C., Chouhan, P. S., and Chaudhary, R. (2008). Evaluation of antifertility activity of Withania somnifera in male albino rats. Fertil. Steril., 90: S18.
Mirjalili, M. H., Moyano, E., Bonfill, M., Cusido, R. M., and Palazón, J. (2009). Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecul., 14(7): 2373-2393.
Munir, N., Mahmood, Z., Shahid, M., Afzal, M. N., Jahangir, M., Ali Shah, S. M., Tahir, I. M., Riaz, M., Hussain, S., Akram, M., and Yousaf, F. (2022). Withania somnifera chemical constituents’ in vitro antioxidant potential and their response on spermatozoa parameters. Dose Response, 20(1): 15593258221074936.
Patil, D., Gautam, M., Jadhav, U., Mishra, S., Karupothula, S., Gairola, S., Jadhav, S., and Patwardhan, B. (2010). Physicochemical stability and biological activity of Withania somnifera extract under real-time and accelerated storage conditions. Planta Med., 76(05): 481-488.
Plotnik, L. (2022). Controlled Environment Production of Ashwagandha (Withania somnifera (L.) Dunal) (Doctoral dissertation, University of Guelph).
Prabu, P. C., Biravi, K., Jarisa, S., Sreepriya, S., and Panchapakesan, S. (2014). Biochemical, pathomorphological and semen characteristics analysis in male wistar rats treated with Withania somnifera root extract. Asian J. Chem., 26(12): 3700-3704.
Rahmati, B., Moghaddam, M. H. G., Khalili, M., Enayati, E., Maleki, M., and Rezaeei, S. (2016). Effect of Withania somnifera (L.) Dunal on sex hormone and gonadotropin levels in addicted male rats. Int. J. Fertil. Steril., 10(2): 239.
Rajalakshmy, M. R., and Geetha, G. (2016). Isolation and identification of withasomnine, withanolides and butein from industrial herbal marc of Withania somnifera (L.) Dunal. J. Nat. Prod. And Res., 7(2): 116-124.
Raut, A. A., Rege, N. N., Tadvi, F. M., Solanki, P. V., Kene, K. R., Shirolkar, S. G., Pandey, S. N., Vaidya, R. A., and Vaidya, A. B. (2012). Exploratory study to evaluate tolerability, safety, and activity of Ashwagandha (Withania somnifera) inlealthy volunteers. J. Ayurveda Integr. Med., 3(3): 111.
Rodriguez-Sánchez, A. J., Meza-Herrera, C. A., De Santiago Miramontes, A., Navarrete-Molina, C., Veliz-Deras, F. G., Ordoñez-Morales, J. Z., Flores-Salas, J. M., and Marin Tinoco, R. I. (2024). Circular Economy, Dairy Cow Feed Leftovers, and Withania somnifera Supplementation: Effects on Black Belly Ram’s Libido, Sperm Quality, Sexual Behavior, and Hemogram Values. Biol., 13(9): 656.
saleem, S., Muhammad, G., Hussain, M. A., Altaf, M., and Bukhari, S. N. A. (2020). Withania somnifera L.: Insights into the phytochemical profile, therapeutic potential, clinical trials, and future prospective. Iran. J. Basic Med. Sci., 23(12): 1501.
Shaikh, N. H., Deshmukh, V. M., and Walvekar, M. V. (2015). Alteration in testicular morphology and sperm count due to Glycowithanolides treatment during aging. Asian J. Pharm. Clin. Res., 8(3): 72-77.
Shukla, K. K., Mahdi, A. A., Mishra, V., Rajender, S., Sankhwar, S. N., Patel, D., and Das, M. (2011). Withania somnifera improves semen quality by combating oxidative stress and cell death and improving essential metal concentrations. Reprod. Biomed. Online., 22(5): 421-427.
Singh, G., Sharma, P. K., Dudhe, R., and Singh, S. (2010). Biological activities of Withania somnifera. Ann. Biol. Res., 1(3): 56-63.
Singh, N., Bhalla, M., de Jager, P., and Gilca, M. (2011). An overview on Ashwagandha: a Rasayana (rejuvenator) of Ayurveda. Afr. J. Tradit. Complement. Altern. Med., 8(5S).
Smith, S. J., Lopresti, A. L., Teo, S. Y., and Fairchild, T. J. (2021). Examining the effects of herbs on testosterone concentrations in men: A systematic review. Adv. Nutr., 12(3): 744-765.
Subbaraju, G. V., Vanisree, M., Rao, C. V., Sivaramakrishna, C., Sridhar, P., Jayaprakasam, B., and Nair, M. G. (2006). Ashwagandhanolide, a bioactive dimeric thiowithanolide isolated from the roots of Withania somnifera. J. Nat. Prod., 69(12): 1790-1792.
Takshak, S., and Agrawal, S. B. (2015). Alterations in metabolite profile and free radical scavenging activities of Withania somnifera leaf and root extracts under supplemental ultraviolet-B radiation. Acta. Physiol. Plant., 37: 1-2.
Trivedi, M. K., Panda, P., Sethi, K. K., and Jana, S. (2017). Metabolite profiling in Withania somnifera roots hydroalcoholic extract using LC/MS, GC/MS and NMR spectroscopy. Chem. Biodivers., 14(3): 1600280.
Udayakumar, R., Kasthurirengan, S., Vasudevan, A., Mariashibu, T. S., Rayan, J. J. S., Choi, C. W., ... and Kim, S. C. (2010). Antioxidant effect of dietary supplement Withania somnifera L. reduce blood glucose levels in alloxan-induced diabetic rats. Plant Foods Hum. Nutr., 65: 91-98.
Uddin, Q., Samiulla, L., Singh, V. K., and Jamil, S. S. (2012). Phytochemical and pharmacological profile of Withania somnifera Dunal: a review. J. Appl. Pharm. Sci., 2(1): 170-175.
Venugopal, S., Padma, M., Rajkumar, M., Seenivasan, N., Saidaiah, P., Sathish, G., Jnanesha, A. C., Kumar, R. S., Kumar, A., Srivastava, J., and Kumari, M. (2022). Comparative investigation of the genetic components of Withania somnifera L. yield and quality traits using multivariate and associations analysis in the Deccan plateau region. Ind. Crops Prod., 188: 115696.
Xu, Y. M., Gao, S., Bunting, D. P., and Gunatilaka, A. L. (2011). Unusual withanolides from aeroponically grown Withania somnifera. Phytochem. Rev., 72(6): 518-522.
Zhang, L., Shi, Y., Yan, M., and Zhang, G. (2022). Modulatory action of withaferin‐A on oxidative damage through regulation of inflammatory mediators and apoptosis via PI3K/AKT signaling pathway in high cholesterol‐induced atherosclerosis in experimental rats. J. Biochem. Mol. Toxicol., 36(10): 23154.
Zhao, J., Nakamura, N., Hattori, M., Kuboyama, T., Tohda, C., and Komatsu, K. (2002). Withanolide derivatives from the roots of Withania somnifera and their neurite outgrowth activities. Chem. Pharm. Bull., 50(6): 760-765.
