Evaluation of the Effect of Chia (Salvia hispanica) Seeds Extract in modulating Fertility Potential, Injury and Histopathological Alterations Induced by Fenitrothion in Male Rats
DOI:
https://doi.org/10.48165/ijar.2025.46.03.15Keywords:
Chia (Salvia hispanica) seeds extract, FSH, Fenitrothion, LH, Testosterone, Sperm count, RatAbstract
The study aimed to evaluate the effect of chia seed extract as a protective against fenitrothion toxicity in male rats. Total forty rats were divided into four groups: the first group was the control group (no treatment), the second group was treated with Chia (Salvia hispanica) seeds at a dose of 400 mg/kg body weight daily, the third group was treated with Fenitrothion at a dose of 100 mg/kg body weight, and the fourth group was treated with both Chia (Salvia hispanica) seeds and fenitrothion. Rats were treated orally for three weeks daily. The results showed changes in the concentration of hormones and sperm characteristics in rats treated with fenitrothion compared to the control group. The exposure to fenitrothion has a noticeable toxic effect on rats through measuring some vital indicators. The role of chia seed extract in reducing lipid oxidation and improving the antioxidant defense system has been proven. We conclude from the above that exposure to fenitrothion has a noticeable toxic effect on rats through measuring some vital indicators, The role of chia seed extract in reducing lipid oxidation and improving the antioxidant defense system has been proven
References
Abdou, H. M., Hammad, M. M., Hussein, H. K., Attia, M. M., & Mohamed, N. A. (2025). Therapeutic effect of vitamin D3 against fipronil-induced oxidative damage, hematological disorders, EPO, RAS abnormalities, and renal impairments in male rats. J. Biosci. Appl. Res., 11(2), 624-645.
Afshar, S., Farshid, A. A., Heidari, R., & Ilkhanipour, M. (2008). Histopathological changes in the liver and kidney tissues of Wistar albino rat exposed to fenitrothion. Tox. Ind. Hea., 24(9), 581-586.
Agarwal, A., Rizwana, Tripathi, A. D., Kumar, T., Sharma, K. P., & Patel, S. K. S. (2023). Nutritional and functional new perspectives and potential health benefits of quinoa and chia seeds. Antioxidants, 12(7), 1413.
Ali, D. A., Essam, N. M., Alzahofy, N. N., Hagras, A. E., El-Kholy, W. M., & Ibrahim, W. M. (2025). Dietary supplementation of brown seaweed (Colpomenia sinuosa) attenuates cadmium hepatotoxicity and oxidative stress in male rats. J. Biosci. Appl. Res., 11(2), 432-448.
Aroniadou-Anderjaska, V., Figueiredo, T. H., de Araujo Furtado, M., Pidoplichko, V. I., & Braga, M. F. (2023). Mechanisms of organophosphate toxicity and the role of acetylcholinesterase inhibition. Toxics, 11(10), 866.
Attia, A. A., Fawzy, M. A., Saleh, S. R., Hamad, H. A., & ElMazoudy, R. (2025). Vitamin C and B12 pretreatment boost the prophylactic mechanism of UVC-induced cardiomyopathy in male rats. J. Biosci. Appl. Res., 11(2), 449-469.
Bhardwaj, J. K., Bikal, P., & Sachdeva, S. N. (2023). Chemotherapeutic drugs induced female reproductive toxicity and treatment strategies. J. Bio. Mol. Tox., 37(7), e23371.
El-Said, S. A., Alballat, S., El-Araby, I. E., Zayed, F. A., & Salama, M. S. (2025). In vivo effects of combined treatment of female mice with Phoenix dactylifera extract and doxorubicin on reducing neighboring cell death during hepatic cancer therapy. J. Biosci. Appl. Res., 11(1), 88-100.
Groswald, A. M., Gripshover, T. C., Watson, W. H., Wahlang, B., Luo, J., Jophlin, L. L., & Cave, M. C. (2023). Investigating the acute metabolic effects of the N-methyl carbamate insecticide, methomyl, on mouse liver. Metabolites, 13(8), 901.
Hasan, A. F., Alankooshi, A. A., Modher, M. N., El-Naggar, S. A., El-Wahsh, H. M., El-Bagoury, A. E., & Kabil, D. I. (2024). Artemisia annua extract ameliorates hepato-renal dysfunctions in obese rats. Ope. Med. Physi., 11(2), 47-65.
Jasim, B. H., & Ali, E. H. (2021). Isolation, extraction, purification, and characterization of fibrinolytic enzyme from Pseudomonas aeruginosa and estimation of the molecular weight of the enzyme. Arch. Ra. Inst., 76(4), 809-820.
Joubert, M. B. V., Ingaramo, P., Oliva, M. E., & D’Alessandro, M. E. (2022). Salvia hispanica L. (chia) seed ameliorates liver injury and oxidative stress by modulating NrF2 and NFκB expression in sucrose-rich diet-fed rats. Food & Function, 13(13), 7333-7345.
Kadhim, A. S., Jasim, B. H., & Ghadir, G. K. (2024). Antibacterial activity of Klebsiella pneumoniae isolated from pneumonia patients. J. Eme. Mede, Traa & Acu Car., 18(6), 47-65.
Lițoiu, A. A., Păucean, A., Lung, C., Zmuncilă, A., & Chiș, M. S. (2025). An overview of buckwheat—a superfood with applicability in human health and food packaging. Plants, 14(14), 2200.
Masood, M. A. B. (2022). Chia seeds as potential nutritional and functional ingredients: A review of their applications for various food industries. J. Nut. Food Sci. Tech., 4(1), 1-14.
McLachlan, R. I., Wreford, N. G., O’Donnell, L., de Kretser, D. M., & Pratis, K. (2002). The endocrine regulation of spermatogenesis: Independent roles for testosterone and FSH. J. Endoc., 172(3), 313–326.
Meijide, F. J., Rey Vázquez, G., Grier, H. J., Lo Nostro, F. L., & Guerrero, G. A. (2016). Development of the germinal epithelium and early folliculogenesis during ovarian morphogenesis in the cichlid fish Cichlasoma dimerus (Teleostei, Perciformes). Acta Zoologica, 97(1), 18-33.
Mihafu, F. D., Kiage, B. N., Kimang’a, A. N., & Okoth, J. K. (2020). Effect of chia seeds (Salvia hispanica) on postprandial glycaemia, body weight and hematological parameters in rats fed a high fat and fructose diet. I. J. Biol. Che. Sci., 14(5), 1752-1762.
Milošević, M. D., Paunović, M. G., Matić, M. M., Ognjanović, B. I., & Saičić, Z. S. (2017). The ameliorating effects of selenium and vitamin C against fenitrothion-induced blood toxicity in Wistar rats. Envir. Toxi. Phar., 56, 204-209.
Mohamed, A. A., El-Megeid, A., Abdel-Aziz, A., & Aboraya, A. O. (2018). Effect of chia seed (Salvia Hispanica L.) on weight reduction of obese rats. Egy. J. Nut. Heal., 13(1), 21-35.
Monageng, E., Offor, U., Takalani, N. B., Mohlala, K., & Opuwari, C. S. (2023). A review on the impact of oxidative stress and medicinal plants on Leydig cells. Antioxidants, 12(8), 1559.
Montes Chani, E. M., Pacheco, S. O., Martínez, G. A., Freitas, M. R., Ivona, J. G., Ivona, J. A., & Pacheco, F. J. (2018). Long-term dietary intake of chia seed is associated with increased bone mineral content and improved hepatic and intestinal morphology in Sprague-Dawley rats. Nutrients, 10(7), 922.
Motyka, S., Koc, K., Ekiert, H., Blicharska, E., Czarnek, K., & Szopa, A. (2022). The current state of knowledge on Salvia hispanica and Salviae hispanicae semen (chia seeds). Molecules, 27(4), 1207.
Sachi, S., Jahan, M. P., Islam, P., Rafiq, K., & Islam, M. Z. (2024). Evaluation of hematoprotective, hepatoprotective, and anti-inflammatory potentials of chia seed (Salvia hispanica L.) extract in rats. Vete. Ani. Sci., 24, 100349.
Salam Hussein, M., Falah Abass, S., Flayyih Hasan, A., G. Al-Dulimi, A., & M. El-Wahsh, H. (2025). Protective role of bee venom (Apis mellifera) against kidney damage in female mice with cancer. Asian Pac. J. Can. Pre., 26(6), 2233-2238.
Savchina, E., Grosso, A. L., Massoner, P., Morozova, K., Scampicchio, M. M., & Ferrentino, G. (2025). From liquid to solid: Exploring techniques, applications, and challenges of structured oils as fat replacements in food formulations. Com. Revie. Fo. Sci. Fo. Saf., 24(2), e70163.
Sharma, P., Singh, R., & Jan, M. (2014). Dose-dependent effect of deltamethrin in testis, liver, and kidney of Wistar rats. Toxi. Interna, 21(2), 131.
Sharma, V., Gupta, P., & Sharma, P. (2023). Recent advancements in novel bioactive peptides and protein hydrolysates isolated from different medicinal plants along with their applications in food and pharmaceutical industries. Inter. J. Pep. Res. Ther., 29(6), 93.
Stein, D. J., Shoptaw, S. J., Vigo, D. V., Lund, C., Cuijpers, P., Bantjes, J., & Maj, M. (2022). Psychiatric diagnosis and treatment in the 21st century: Paradigm shifts versus incremental integration. Wor. Psyc., 21(3), 393-414.
Yahya, A., Adil Obaid, W., Mohammed Hameed, O., & Hasan, A. F. (2024). Histopathological and immunohistochemical
studies on the effects of silver oxide nanoparticles (AgNPs) on male rats’ liver. Journal of Bioscience and Applied Research, 10(3), 392-398. doi:10.21608/jbaar.2024.298240.1054
