Effect of Fetal Calf Serum (FCS) Versus Polyvinylpyrrolidine (PVP) on in vitro Maturation of Oocytes Retrieved by Ovum Pick-up (OPU) in Sahiwal Cows
DOI:
https://doi.org/10.48165/ijar.2025.46.03.4Keywords:
Cumulus Oocyte Complexes (COCs), Fetal Calf Serum (FCS),, In Vitro Maturation (IVM), Ovum Pick-up (OPU), Polyvinylpyrolidine (PVP)Abstract
The experiment was conducted to compare the effect of supplementation of fetal calf serum (FCS) versus polyvin ylpyrrolidine (PVP) on in vitro maturation (IVM) of the bovine oocytes collected by ovum pick-up (OPU) method. Cumulus oocyte complexes (COC’s) obtained by follicular aspiration having at least a complete layer of cumulus cells were randomly divided into two groups containing 10 replicates each of 10-12 oocytes. In vitro maturation of the COC’s supplementation of 5% FCS showed (Mean±Std. Error, %) higher full expansion of cumulus cells (9.20±0.64, 90.20) in comparison to 1% PVP (8.5±0.45, 81.73), though non-significant. On the other hand, COC’s showing intermedi ate expansion were significantly low (p<0.05) in maturation media (Mean±Std. Error, %) supplemented with 5% FCS (0.40±0.21, 3.9) in comparison to 1% PVP (1.30± 0.30, 12.50). Higher maturation rate in media supplemented with FCS might be due to growth factors, nutrients, hormones and antioxidants present in it. FCS contains number of growth fac tors that play an important role in regulation of oocyte maturation, particularly via cumulus cells. IVM media containing 5% FCS provides a favourable environment in comparison to 1% PVP for in vitro maturation of bovine oocytes obtained though ovum pick-up.
References
Abe, H., & Hoshi, H. (2003). Evaluation of bovine embryos produced in high performance serum-free media. J. Reprod. Dev., 49(3), 193–202.
Abolfazl, S., Mohammad, A. A., Ebrahim, A., Hassan, N., Morteza, M., & Banafsheh, H. (2012). The effect of macromolecule source and type of media during in vitro maturation of sheep oocytes on subsequent embryo development. J. Reprod. Infertil., 13(1), 13–19.
Arias, M. E., Vargas, T., Gallardo, V., Aguila, L., & Felmer, R. (2022). Simple and efficient chemically defined in vitro maturation and embryo culture system for bovine embryos. Animals, 12(21), 3057.
Choi, B. H., Park, B. Y., Kong, R., Son, M. J., Park, C. S., Shin, N. H., & Kong, I. K. (2019). Effect of serum and serum-free media on the developmental competence of OPU derived bovine IVP embryo. J. Anim. Reprod. Biotechnol., 34(4), 305–310.
Chung, J. T., Tosca, L., Huang, T. H., Xu, L., Niwa, K., & Chian, R. C. (2007). Effect of polyvinylpyrrolidone on bovine oocyte maturation in vitro and subsequent fertilization and embryonic development. Reprod. Biomed. Online, 15(2), 198–207.
Franca, P. H., Santos, M. V., Oliveira, G. P., Azevedo, A., & Pereira, A. (2020). Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes. Rev. Colomb. Cienc. Pecu., 33(3), 172–181.
Gstraunthaler, G. (2003). Alternatives to the use of fetal bovine serum: Serum-free cell culture. ALTEX, 20(4), 275–281.
Imtiyaz, N., Brar, P. S., Singh, N., & Malik, V. S. (2021). Effect of two different FSH doses on superovulatory response and embryo production in Sahiwal cows. Indian J. Anim. Reprod., 42(2), 56–59.
Kim, H. S., Lee, G. S., Hyun, S. H., Lee, S. H., Nam, D. H., Jeong, Y. W., ... & Hwang, W. S. (2004). Improved in vitro development of porcine embryos with different energy substrates and serum. Theriogenology, 61(7–8), 1381–1393.
Neglia, G., Gasparrini, B., di Brienza, V. C., Di Palo, R., Campanile, G., Presicce, G. A., & Zicarelli, L. (2003). Bovine and buffalo invitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology, 59(5–6), 1123–1130.
Prasad, S., Singh, B., Singhal, S., Khan, F. A., Prasad, J. K., & Gupta, H. P. (2013). Production of the first viable ovum pick-up and in vitro embryo produced (OPU–IVEP) buffalo calf in India. Asian Pac. J. Reprod., 2(2), 163–165.
Puri, G., Chaudhary, S. S., Singh, V. K., & Sharma, A. K. (2015). Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro. Vet. World, 8(2), 143–146.
Rzucidlo, S. J., Gibbons, J., & Stice, S. L. (2001). Comparison by restriction fragment differential display RT-PCR of gene expression pattern in bovine oocytes matured in the presence or absence of fetal calf serum. Mol. Reprod. Dev., 59, 90–96.
Santos, M. V. O., Queiroz, N. L. B., Borges, A. A., & Pereira, A. F. (2017). Influence of commercially available follicle stimulating hormone on the in vitro maturation of bovine oocytes. Semin. Cienc. Agrar., 38(3), 1393–1402.
Singh, N., Dhaliwal, G. S., Malik, V. S., Singhal, S., Honparkhe, M., & Brar, P. S. (2016). Effect of aspiration pressure on recovery and quality of oocyte following ovum pick-up in buffaloes. Indian Vet. J., 93(10), 37–40.
Singh, N., Dhaliwal, G. S., Brar, P. S., Singhal, S., & Malik, V. S. (2021). In vitro maturation of buffalo oocytes in serum-free media supplemented with growth factors. Haryana Vet., 60, 1–4.
Singh, N., Mavi, G. K., Kumar, A., & Malik, V. S. (2023). Effect of 2-day versus 3-day superstimulation protocol on in vivo maturation of OPU derived oocytes in buffaloes. Indian J. Anim. Reprod., 44(1), 17–22.
Singh, S., Prasad, S., Gupta, H. P., Singhal, S., Gupta, A. K., & Kumar, A. (2012). Isolation and characterization of oviduct-specific glycoproteins from ampulla and isthmus parts of cyclic and acyclic buffalo for studying differential microenvironment. Appl. Biochem. Biotechnol., 166, 1814–1830.
Singhal, S., Singh, N., Malik, V. S., Kumar, A., & Brar, P. S. (2017). A preliminary study on superstimulatory response using low dose FSH and subsequent embryo transfer in Sahiwal cattle during summer season. Indian J. Anim. Reprod., 38(2), 37–39.
Sobti, D. (2018). Assessment of developmental competence of in vitro and in vivo matured oocytes retrieved by ovum pick up in buffaloes (Bubalus bubalis). [Thesis, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana].
Vettical, B. S. (2016). Effect of fetal calf serum and mid estrus cow serum on in vitro maturation and fertilization of oocytes from crossbred cattle in the tropics. Int. J. Appl. Sci. Biotechnol., 4(3), 308–310.
