Protection of cell membrane of buffalo spermatozoa during cryopreservation

Abstract

Cell membrane of buffalo spermatozoa was substantially damaged in the egg yolk tris dilutor during cryopreservation as
evidenced by greater release of transaminases and dehydrogenases enzymes. Insulin and foetal calf serum were found to protect the
sperm cell membrane as evinced by reduced leakage of these enzymes and imporved level of motile, live and normal spermatozoa
during cryopreservation.